Objective:To evaluate in vitro antioxidant and apoptotic activities of Crperus rotundas(C.rotundus).Methods:The phytochemical study and the antioxidant activities of both methanol and aqueous extracts from C.rotundus aerial part were determined.In addition,these extracts were also investigated for their cytotoxic and apoptotic activities.The major compound of the methanol extract was isolated.Both metlianol and aqueous extracts(300,150,and 50μg/mL)were evaluated for their antioxidant activity by the xanthine/xanthine oxidase assay system.However,16,8,and 4 mg/mL of each extract were tested to investigate their OH.formation scavenging potential.Aqueous extract(800,400.and 200μg/mL)and melhunol extract(350,175,and 88μg/mL)were tested against lipid peroxidation,induced by 75μM H_2O_2,The cytotoxicity(by MTT assay)and cell DNA fragmentation of both extracts were evaluated Inwards K562 and L1210 cell lines.The major compound was obtained from the butanol fraction of methanol extract and its structure was determined by KMN spectroscopic analysis.Results:The methanol and aqueous extracts showed respectively,88%and 19%inhibition of xanthine oxidase activity.Vet.the same extracts inhibited lipid peroxidation by 61.5%and 42.0%.respectively.Roth extracts inhibited OH.formation by 27.1%and 25.3%,respectively.Only methanol cxtract induced DNA degradation.Orientin was determined as the major compound isolated from the butanol fraction of metlianol extract.Conclusions:It appears that C.rotundus extracts exhibit a potential use as a natural antioxidant and an apoptosis inducer. Objective: To evaluate in vitro antioxidant and apoptotic activities of Crperus rotundas (C. rotundus). Methods: The phytochemical study and the antioxidant activities of both methanol and aqueous extracts from C. rotundus aerial part were determined. In addition, these extracts were also investigated for their cytotoxic and apoptotic activities. The major compound of the methanol extract was isolated. Both metlianol and aqueous extracts (300, 150, and 50 μg / mL) were evaluated as their antioxidant activity by the xanthine / xanthine oxidase assay system. 8 and 4 mg / mL of each extract were tested to investigate their OH.formation scavenging potential. Aqueous extract (800, 400 and 200 μg / mL) and melhunol extract (350, 175 and 88 μg / mL) were tested against lipid peroxidation, induced by 75 μM H 2 O 2, The cytotoxicity (by MTT assay) and cell DNA fragmentation of both extracts were evaluated as Inwards K562 and L1210 cell lines. The major compound was obtained from the butanol fraction of methanol extract and Results: The methanol and aqueous extracts were 88% and 19% inhibition of xanthine oxidase activity. Vet. the same extracts inhibited lipid peroxidation by 61.5% and 42.0% respectively. OH.formation by 27.1% and 25.3%, respectively.Only methanol cxtract induced DNA degradation. Orrinin was determined as the major compound isolated from the butanol fraction of metlianol extract. Conclusions: It appears that C.rotundus extract exhibit a potential use as a natural antioxidant and an apoptosis inducer.