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目的利用高通量测序,研究牙周炎患牙根面微生物的群落结构,为牙周炎的进展研究提供实验依据。方法选取同一牙周炎患者的6颗Ⅲ度松动患牙,分别刮取根颈(RN组)、根中(RM组)、根尖(RT组)菌斑及组织,利用Illumina Miseq测序平台,对16SrRNA V3V4区进行双端测序;通过Mothur、Qiime和SPSS等软件分析根面不同部位微生物的群落结构及差异。结果主坐标分析(PCoA)显示,RM与RT组微生物群落结构较接近,但二者与RN组有明显区别;3组共检出13个菌门,优势菌门(丰度>1%)共7个;184种菌属中检出优势菌属(丰度>1%)29个,其中拟杆菌门_[G-6]及消化链球菌科_[XI][G-4]丰度会随着采样部位的深入而增加(P<0.05),而普雷沃菌属、月形单胞菌属、棒状杆菌属及欧森氏菌属的丰度随着采样部位的深入而减少(P<0.05)。结论牙周炎患牙根面微生物群落结构具有一定的部位特异性。
Objective To study the community structure of root-surface microorganisms in periodontitis by high-throughput sequencing and provide experimental basis for the study of the progression of periodontitis. Methods Six teeth with Ⅲ degree loosening in the same periodontitis were selected and the plaque and tissue of the root neck (RN group), root (RM group) and root tip (RT group) were scraped respectively. Illumina Miseq sequencing platform The 16S rRNA V3V4 region was double-stranded sequenced and the community structures and differences of the microorganisms in different parts of the root surface were analyzed by software Mothur, Qiime and SPSS. Results PCoA analysis showed that the microbial communities in RM and RT groups were similar, but there was a significant difference between them and RN group. Thirteen Fungicides and Fungicides (abundance> 1%) were detected in 3 groups Among them, 29 strains of dominant genus (abundance> 1%) were detected in 184 species, of which Bacteroidetes _ [G-6] and Peptostreptococci _ [XI] [G-4] (P <0.05). However, the abundance of Prevotella, Glomonella, Corynebacterium and the genus of the genus Aspergill decreased with the depth of the sampling site (P <0.05) <0.05). Conclusion The periodontitis with root-like surface microbial community structure has a certain site-specific.