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目的:检测氯沙坦干预下、槟榔碱诱导的人脐静脉内皮细胞(HUVECs)表达α-SMA的情况。方法:用不同浓度槟榔碱刺激HUVECs,采用免疫细胞印迹法检测各组HUVECs中α-SMA的表达。再以固定槟榔碱浓度诱导HUVECs,不同浓度氯沙坦干预,检测各组HUVECs中α-SMA的表达。结果:槟榔碱可诱导HUVECs表达α-SMA,经氯沙坦干预后α-SMA的表达受到一定抑制。结论:HUVECs经槟榔碱刺激后可转化为表达α-SMA的肌成纤维细胞。血管紧张素Ⅱ受体拮抗剂氯沙坦可部分抑制这种转化,提示血管紧张素Ⅱ及其受体参与了槟榔碱诱导的内皮-间充质转化。
OBJECTIVE: To detect the expression of α-SMA in arecoline-induced human umbilical vein endothelial cells (HUVECs) treated with losartan. Methods: HUVECs were stimulated with different concentrations of arecoline, and the expression of α-SMA in each group of HUVECs was detected by immunocytometry. HUVECs were induced by fixed arecoline concentration and Losartan at different concentrations. The expression of α-SMA in HUVECs of each group was detected. RESULTS: Arecoline induced the expression of α-SMA in HUVECs, and the expression of α-SMA was inhibited by losartan. CONCLUSION: HUVECs can be transformed into α-SMA-expressing myofibroblasts by arecoline stimulation. Losartan, an angiotensin II receptor antagonist, partially inhibited this transformation, suggesting that angiotensin II and its receptor are involved in arecoline-induced endothelial-mesenchymal transition.