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为了得到基于相位检测的表面等离子体共振(SPR)生物传感器的高检测精度,建立了这种传感器的5层膜结构的数学模型,利用Matlab系统地进行了耦联层、传感层等对所检测相位变化影响的数值模拟,确定了一组优化参数。模拟结果表明,相位检测对传感层的折射率具有选择性。获得较高的分辨率的条件是:耦联层厚度应小于150nm,优选值是50nm;不生成传感亚层时,传感基层的厚度应控制在200nm左右;当生成传感亚层时,传感层(包括基层和亚层)的总体厚度须根据反应物分子的特异性系统考虑。
In order to obtain the high detection accuracy of the SPR biosensor based on phase detection, a mathematical model of the five-layer membrane structure of the sensor was established. The coupling layer, sensing layer and so on were systematically analyzed by Matlab A numerical simulation of the effect of phase changes was detected, and a set of optimization parameters was determined. Simulation results show that phase detection is selective to the refractive index of the sensing layer. The conditions for obtaining a higher resolution are: the thickness of the coupling layer should be less than 150 nm and the preferred value is 50 nm; when the sensing sublayer is not generated, the thickness of the sensing substrate should be controlled at about 200 nm; when generating the sensing sublayer, The overall thickness of the sensing layer (including the base layer and sub-layer) must be based on the specific system of reactant molecules.