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应用聚合酶链反应（ＰＣＲ）技术对石蜡包埋的６０例口腔鳞状细胞癌（以下简称鳞癌）和４０例正常口腔粘膜标本进行ＥＢ病毒的ＤＮＡ（ＥＢＶ－ＤＮＡ）检测，探讨ＥＢＶ与口腔鳞癌间的关系。口腔鳞癌中高分化４０例，中分化１０例，低分化１０例。结果显示６０例口腔鳞癌中２０例ＥＢＶ－ＤＮＡ检测阳性，检出率为３３．３％，其中高分化１３例，中分化３例，低分化４例；正常组４例检测阳性，检出率为１０％。经χ２检验χ２＝７．１６，两组间差异有显著性（Ｐ＜０．０１），同时对各病损部位、年龄组和组织分化间的ＥＢＶ－ＤＮＡ检出率进行统计学处理，差异均无显著性（Ｐ＞０．０５）。该研究结果提示口腔鳞状上皮组织的ＥＢＶ感染极可能参与了口腔粘膜的癌变过程。
Application of polymerase chain reaction (PCR) to the detection of EBV DNA (EBV-DNA) in paraffin-embedded 60 cases of oral squamous cell carcinoma (hereinafter referred to as squamous cell carcinoma) and 40 cases of normal oral mucosa to investigate EBV and oral cavity The relationship between squamous cell carcinoma. Oral squamous cell carcinoma was well differentiated in 40 cases, moderately differentiated in 10 cases, and poorly differentiated in 10 cases. The results showed that 20 out of 60 cases of oral squamous cell carcinoma were positive for EBV-DNA, with a detection rate of 33.3%. Among them, 13 were well-differentiated, 3 were moderately differentiated, and 4 were poorly differentiated; 4 were positive in the normal group and detected. The rate is 10%. The χ2 test χ 2 = 7.16, the difference between the two groups was statistically significant (P <0.01). At the same time, the EBV-DNA detection rate of each lesion site, age group and tissue differentiation was statistically treated. No significant (P>0.05). The results suggest that EBV infection in oral squamous epithelium may be involved in the carcinogenesis of oral mucosa.