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应用聚合酶链反应(PCR)技术对石蜡包埋的60例口腔鳞状细胞癌(以下简称鳞癌)和40例正常口腔粘膜标本进行EB病毒的DNA(EBV-DNA)检测,探讨EBV与口腔鳞癌间的关系。口腔鳞癌中高分化40例,中分化10例,低分化10例。结果显示60例口腔鳞癌中20例EBV-DNA检测阳性,检出率为33.3%,其中高分化13例,中分化3例,低分化4例;正常组4例检测阳性,检出率为10%。经χ2检验χ2=7.16,两组间差异有显著性(P<0.01),同时对各病损部位、年龄组和组织分化间的EBV-DNA检出率进行统计学处理,差异均无显著性(P>0.05)。该研究结果提示口腔鳞状上皮组织的EBV感染极可能参与了口腔粘膜的癌变过程。
Application of polymerase chain reaction (PCR) to the detection of EBV DNA (EBV-DNA) in paraffin-embedded 60 cases of oral squamous cell carcinoma (hereinafter referred to as squamous cell carcinoma) and 40 cases of normal oral mucosa to investigate EBV and oral cavity The relationship between squamous cell carcinoma. Oral squamous cell carcinoma was well differentiated in 40 cases, moderately differentiated in 10 cases, and poorly differentiated in 10 cases. The results showed that 20 out of 60 cases of oral squamous cell carcinoma were positive for EBV-DNA, with a detection rate of 33.3%. Among them, 13 were well-differentiated, 3 were moderately differentiated, and 4 were poorly differentiated; 4 were positive in the normal group and detected. The rate is 10%. The χ2 test χ 2 = 7.16, the difference between the two groups was statistically significant (P <0.01). At the same time, the EBV-DNA detection rate of each lesion site, age group and tissue differentiation was statistically treated. No significant (P>0.05). The results suggest that EBV infection in oral squamous epithelium may be involved in the carcinogenesis of oral mucosa.