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目的 探讨三氧化二砷诱导表达带 3蛋白膜段结构域K5 6 2细胞凋亡的作用。方法 将纯化的表达载体pYD1 mdb3转染K5 6 2细胞 ,WesternBlot检定有带 3蛋白膜段结构域的表达 ;通过光学显微镜、DNA电泳和线粒体膜电位的测定 ,观察三氧化二砷对表达带 3蛋白膜段结构域的K5 6 2细胞凋亡的影响。结果 表达带 3蛋白膜段结构域的K5 6 2细胞的上述凋亡特征均比未表达带 3蛋白的K5 6 2细胞明显。结论 表达带 3蛋白膜段结构域的K5 6 2细胞对三氧化二砷更敏感
Objective To investigate the effect of arsenic trioxide on the apoptosis of K562 cells with 3 protein membrane-associated domain. Methods The purified expression vector pYD1 mdb3 was transfected into K562 cells. Western Blot was used to detect the expression of membrane protein with band 3. By optical microscopy, DNA electrophoresis and mitochondrial membrane potential measurement, the effect of arsenic trioxide on the expression of membrane protein 3 Domain of K562 cells apoptosis. Results The above apoptotic characteristics of K562 cells expressing the 3 protein membrane domain were all significantly higher than those of K562 cells without 3 protein. Conclusion K562 cells expressing the 3-protein membrane-segment domain are more sensitive to arsenic trioxide