作者报道了一种简称为M. V. 法的利什曼无鞭毛体体内培养技术用于诊断皮肤利什曼病的试验工作。将4×10~6小鼠肉瘤细胞和2×10~6前鞭毛体一起注射到BABL/c小鼠的腹腔内,10～15天之后即可在形成的腹水中生成数以千万计的细胞和无鞭毛体。经碾碎并被一种梯 The authors report the use of Leishman’s mastoid body in vitro culture assay referred to as M. V. method for the diagnosis of cutaneous leishmaniasis. 4 × 10 ~ 6 mouse sarcoma cells were injected together with 2 × 10 ~ 6 promastigotes into the peritoneal cavity of BABL / c mice, and 10 to 15 days later, tens of millions of Cells and amastigotes. Milled and by a ladder