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一、白细胞粘附抑制试验的近况白细胞粘附抑制(LAI)试验是Halliday等于1971年首先报告的,他们发现肿瘤大鼠的腹腔细胞与相应肿瘤抗原共同培养后,粘附于玻璃器皿表面的能力明显减弱,认为这是一种特异性细胞免疫反应现象。以后,随着试验方法的改进和实验研究的成功,LAI试验引起了许多学者的注意。1978年,在纽约举行了国际性LAI试验讨论会,一致认为LAI试验是一项简便易行、敏感性和特异性较好的细胞免疫检测方法。 LAI试验的方法很多,最常用的是血细胞计数板法、试管法和微量培养板法,其具体操作程序国内
First, the leukocyte adhesion inhibitory test of the recent status of leukocyte adhesion inhibition (LAI) test is Halliday equal to the first report in 1971, they found that tumor cells of the rat’s abdominal cavity and the corresponding tumor antigen cocultured, the ability to adhere to the surface of glassware Obviously weakened, think this is a specific cellular immune response phenomenon. Later, with the improvement of experimental methods and the success of experimental research, the LAI test attracted the attention of many scholars. In 1978, an international LAI trial seminar was held in New York. It was agreed that the LAI test is a simple, sensitive, and specific method for cell immunoassay. There are many methods for the LAI test. The most common ones are the blood cell count plate method, the test tube method, and the microplate method. The specific operating procedures are