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本文采用氢气清除法测定胃粘膜血流以及皮下注射大剂量辣椒素使传入神经“去神经”的技术，观察了大鼠胃内灌注蛋白胨引起胃酸分泌过程中胃粘膜血流量（ｇａｓｔｒｉｃ ｍｕｃｏｓａｌ ｂｌｏｏｄ ｆｌｏｗ，ＧＭＢＦ）的变化以及传入神经在这一效应中的作用。结果表明：（１）胃内灌注６％蛋白胨引起胃酸分泌时，ＧＭＢＦ增加；（２）预先用大剂量辣椒素消除传入神经作用可阻断胃肉蛋白胨引起的ＧＭＢＦ增加效应，并部分阻断胃酸分泌反应；（３）预先静脉注射一氧化氮（ｎｉｔｒｉｃ ｏｘｉｄｅ，ＮＯ）生物合成阻断剂Ｌ－ｎｉｔｒｏ－Ｌ－ａｒｇｉｎｉｎｅｍｅｔｈｙｌ ｅｓｔｅｒ（Ｌ－ＮＡＭＥ）可阻断胃肉蛋白胨引起的胃粘膜血流增多效应，同时胃酸分泌增多效应减弱；（４）预先静脉注射ＮＯ生物合成前体Ｌ－精氨酸（Ｌ－ａｒｇｉｎｉｎｅ，Ｌ－ＡＲＧ）可以翻转Ｌ－ＮＡＭＥ对ＧＭＢＦ和胃酸分泌的阻断作用；（５）预先静脉注射胆碱能Ｍ受体阻断剂阿托品可以完全阻断胃内蛋白胨引起的胃酸分泌增加效应，但仅部分阻断ＧＭＢＦ的增加效应。实验结果提示：辣椒素敏感传入神经成分和ＮＯ参与胃内蛋白胨引起的胃酸分泌及胃粘膜血流增多效应，而胃粘膜血流增多效应可以通过胆碱能成分和非胆碱能成?
In this paper, hydrogen scavenging method for the determination of gastric mucosal blood flow and subcutaneous injection of high-dose capsaicin so that the afferent nerve “denervation” technology was observed in the rat stomach peptone caused gastric acid secretion during gastric mucosal blood flow (gastric mucosal blood flow , GMBF) changes and the role of afferent nerve in this effect. The results showed that: (1) GMBF increased when gastric intragastric instillation of 6% peptone caused gastric acid secretion; (2) pretreatment with high dose of capsaicin to eliminate afferent nerve blocked the effect of gastric peptone-induced GMBF increase and partial block (3) Preinjection of nitric oxide (NO) biosynthesis blocker L-nitro-L-arginine methyl ester (L-NAME) can block gastric peptone-induced increase of gastric mucosal blood flow (4) Pretreatment with L-arginine (L-ARG), a precursor of NO biosynthesis, could reverse the blocking effect of L-NAME on GMBF and gastric acid secretion; ( 5) Pretreatment with intravenous cholinergic M blocker atropine completely blocked the effect of gastric peptone on gastric acid secretion, but only partially blocked the increase of GMBF. The experimental results suggest that capsaicin-sensitive afferent nerve components and NO participate in gastric acid secretion caused by peptone in the stomach and gastric mucosal blood flow increase effect, and gastric mucosal blood flow increase effect can be caused by cholinergic components and non-cholinergic?