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[Object] To optimize the isolation and purification process of puerarin by macroporous resin.[Method] Puerarin was isolated by static and dynamic adsorption-desorption method and determined by HPLC.[Result] HPD-800 macroporous resin had the best effect on isolating puerarin,the optimum purification conditions were as follows:sampling concentration of 0.2 mg/ml,pH of 1.0,flow rate of 2.0 ml/min;70% ethanol as eluent,volume of 5 BV,flow rate of 1.5 ml/min.The yield of puerarin and the purity were up to 91.02% and 96.13% respectively.[Conclusion] HPD-800 macroporous resin had good effect on isolation and purification of puerarin with the advantages of simple and short production cycle,which would enjoy good application in practical production.
[Object] To optimize the isolation and purification process of puerarin by macroporous resin. [Method] Puerarin was isolated by static and dynamic adsorption-desorption method and determined by HPLC. [Result] HPD-800 macroporous resin had the best effect on isolating puerarin , the optimum purification conditions were as follows: sampling concentration of 0.2 mg / ml, pH of 1.0, flow rate of 2.0 ml / min; 70% ethanol as eluent, volume of 5 BV, flow rate of 1.5 ml / min. of puerarin and the purity were up to 91.02% and 96.13% respectively. [Conclusion] HPD-800 macroporous resin had good effect on isolation and purification of puerarin with the advantages of simple and short production cycle, which would enjoy good application in practical production .