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目的利用LC-MS/MS,建立同时测定三七茎基总皂苷中人参皂苷Rg1和Rb1含量的分析方法。方法色谱柱:BDS HYPERSUL C18柱(150 mm×2.1 mm,5μm);流动相:乙腈-水(梯度洗脱);柱温:30℃;质谱条件:采用负离子多反应监测方法(MRM)测试,用于定量分析的对照品离子对分别为:人参皂苷Rg1 m/z 799.6→475.5;人参皂苷Rb1 m/z1 107.9→783.7;内标物紫杉醇m/z 852.5→525.3。结果人参皂苷Rg1、人参皂苷Rb1的线性范围分别为0.173～17.3μg.mL-1和0.159～16.0μg.mL-1,精密度和准确度等均符合样品分析的要求。结论该法准确、灵敏、特异性强,适用于三七茎基总皂苷及其制剂中人参皂苷Rg1、Rb1浓度的同时测定。
OBJECTIVE To establish a method for the simultaneous determination of ginsenoside Rg1 and Rb1 in Panax notoginseng saponins by LC-MS / MS. Methods Column: BDS HYPERSUL C18 column (150 mm × 2.1 mm, 5 μm); mobile phase: acetonitrile-water (gradient elution); column temperature: 30 ℃; mass spectrometry conditions: negative ion multiple reaction monitoring (MRM) The standard ion pairs used for quantitative analysis were: ginsenoside Rg1 m / z 799.6 → 475.5; ginsenoside Rb1 m / z1 107.9 → 783.7; and the internal standard paclitaxel m / z 852.5 → 525.3. Results The linear ranges of ginsenoside Rg1 and ginsenoside Rb1 were 0.173 ~ 17.3μg.mL-1 and 0.159 ~ 16.0μg.mL-1, respectively. The precision and accuracy of this method met the requirements of sample analysis. Conclusion The method is accurate, sensitive and specific and suitable for the simultaneous determination of ginsenoside Rg1 and Rb1 in Panax notoginseng saponins and its preparations.