硒是机体必须的微量元素,参与机体多种酶的合成,可提高机体免疫力,调节机体代谢,具有清除自由基和高抗氧化活性的功效,被称为“天然解毒剂”。研究表明~([1]),硒对过量氟化物导致小鼠成釉细胞DNA损伤的具有拮抗作用。本文通过建立体外培养的小鼠成釉细胞染氟及染硒模型对此进行研究,为氟中毒防治提供依据。1材料与方法1.1细胞、材料及仪器由山东省潍坊医学院赠送的小鼠成釉细胞,基因表达鉴定显示具有特定成簇蛋白、 Selenium is a necessary trace element of the body, involved in the synthesis of a variety of enzymes in the body, can improve the body immunity, regulate body metabolism, with the effect of scavenging free radicals and high antioxidant activity, known as “natural antidote ”. Studies have shown that ~ ([1]), selenium on fluoride-induced mouse ameloblast DNA damage has antagonistic effect. In this paper, we established a model of in vitro cultured ameloblasts stained with fluoride and selenium, and provided the basis for the prevention and treatment of fluorosis. 1 Materials and Methods 1.1 Cells, Materials and Instruments Mouse ameloblasts donated by Weifang Medical College of Shandong Province, the gene expression identification showed a specific cluster of proteins,