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作者用体外培养方法观察了一些包括已知有效的及有关化合物对曼氏血吸虫童虫、虫龄3周的血吸虫和成虫的作用。培养液系用Earle的乳白蛋白/新生牛犊血清(1∶1)。将上述血吸虫置于2毫升培养液中培养。所试药物皆溶于二甲基亚砜,加至培养液的溶媒最大量为10微升。血吸虫于用加药的培养液培养24、48及72小时后,用显微镜观察其活动,并根据虫体活动或死亡情况评价药效。结果,血吸虫童虫对药物的敏感性较虫龄为3周的血吸虫及成虫的为大。在所筛选的400个化合物中,当药物浓度为1ppm时,有杀童虫作用的为80个(20%)。用20ppm浓度分别观察200个及125个化合物对虫龄为3周
In vitro culture methods were used to observe some of the known and effective and related compounds on Schistosoma mansoni schistosomiasis, three weeks old worm schistosome and adult role. Earle’s lactalbumin / neonatal calf serum (1: 1) was grown. The above schistosomes were placed in 2 ml of culture medium. The drugs tested were dissolved in dimethyl sulfoxide, the maximum amount of medium added to the culture medium was 10 microliters. Schistosoma japonicum was cultured for 24, 48 and 72 hours in dosing culture medium. The activity of schistosomiasis was observed with a microscope and the efficacy was evaluated according to the activity or death of the parasites. As a result, schistosomiasis was more susceptible to drugs than schistosomes and adults at 3 weeks of age. Among the 400 compounds screened, there were 80 (20%) mice with the effect of killing the worms when the drug concentration was 1 ppm. 200 and 125 compounds were observed for 20 weeks at a 20 ppm concentration for three weeks