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目的研究在中枢神经系统（ＣＮＳ）的炎症性疾病中，作为构成血脑屏障结构的组成部分之一，可引起白细胞浸润的星形细胞分泌趋化因子的影响因素。方法通过星形细胞体外培养，经不同细胞因子刺激，用原位杂交检测成年大鼠星形细胞β－家系趋化因子的ｍＲＮＡ表达。结果ＩＦＮ－γ可引起ＭＣＰ－１，ＲＡＮＴＥＳ，ＭＩＰ－１α和ＭＩＰ－１β的ｍＲＮＡ表达；ＴＮＦ－α可引起ＲＡＮＴＥＳ和ＭＩＰ－１β的ｍＲＮＡ表达；ＬＰＳ可引起ＭＩＰ－１α和ＭＩＰ－１β的ｍＲＮＡ表达。ＴＧＦ－β和ＩＬ－１０下调由ＬＰＳ引起的ＭＣＰ－１和ＭＩＰ－１α和ＭＩＰ－１βｍＲＮＡ表达。ＴＧＦ－β和ＩＬ－１０可抑制由ＴＮＦ－α引起的ＭＣＰ－１和ＲＡＮＴＥＳ的ｍＲＮＡ表达。ＩＬ－１０还可下调由ＴＮＦ－α引起的ＭＩＰ－１βｍＲＮＡ表达。结论成年大鼠体外试验中的星形细胞可由ＬＰＳ和前炎症因子刺激，产生β－家系趋化因子ｍＲＮＡ的表达。而调节因子如ＴＧＦ－β和ＩＬ－１０可抑制由ＩＦＮ－γ、ＴＮＦ－α和ＬＰＳ引起的β－家系趋化因子的ｍＲＮＡ表达。
Objective To investigate the factors that can cause leukocyte infiltration of chemokines by leukocyte infiltration in inflammatory diseases of the central nervous system (CNS) as one of the components that make up the blood-brain barrier structure. Methods Astrocytes were cultured in vitro and stimulated by different cytokines. The mRNA expression of β-family chemokines in adult rat astrocytes was detected by in situ hybridization. Results IFN-γ induced the mRNA expression of MCP-1, RANTES, MIP-1α and MIP-1β; TNF-α induced the mRNA expression of RANTES and MIP-1β; LPS induced the mRNA expressions of MIP-1α and MIP-1β . TGF-β and IL-10 down-regulated MCP-1 and MIP-1α and MIP-1β mRNA expression by LPS. TGF-β and IL-10 inhibit the mRNA expression of MCP-1 and RANTES caused by TNF-α. IL-10 also down-regulates MIP-1β mRNA expression caused by TNF-α. Conclusion Astrocytes in adult rat in vitro experiments can be stimulated by LPS and proinflammatory cytokines, resulting in the expression of β-family chemokine mRNA. Regulatory factors such as TGF-β and IL-10 inhibited the mRNA expression of β-family chemokines induced by IFN-γ, TNF-α and LPS.