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目的:研究缓衰方对纤维连接蛋白(FN)、转化生长因子β_2(TGF-β_2)在5/6肾切除大鼠肾小球中的含量的影响。方法:Wistar 大鼠5/6肾切除造模,随机分为对照组、缓衰方组,观察实验开始、第6周及第12周血肌酐(SCr)、24 h 尿蛋白定量变化,以及免疫组化加图像分析检测 FN、TGF-β_2的相对含量。结果:动物经5/6肾切除后,SCr、24 h 尿蛋白定量增加,经缓衰方灌胃12周,SCr、24 h 尿蛋白定量减少,与对照组比较,差异有显著性(P<0.05);免疫组化显示,缓衰方组 FN、TGF-β_2的相对含量较对照组明显降低,两组比较,差异亦有显著性(P<0.05)。结论:缓衰方通过抑制过高 TGF-β_2水平,而减少细胞外基质成分 FN 在肾小球的积聚,延缓肾衰进展速度,保护残肾功能。
Objective: To investigate the effects of slow-reducing recipes on the content of fibronectin (FN) and transforming growth factor β 2 (TGF-β 2) in glomeruli of 5/6 nephrectomized rats. METHODS: Wistar rats were modeled after 5/6 nephrectomy and randomly divided into control group and chronic failure group. The serum creatinine (SCr), 24-hour urine protein quantification, and immunity were observed at the beginning of the experiment, 6 weeks and 12 weeks. The relative content of FN and TGF-β2 was detected by histological plus image analysis. RESULTS: After 5/6 nephrectomy in animals, the urinary protein of SCr and 24 h increased. After being fed for 12 weeks, the quantitation of urinary protein in SCr and 24 h decreased. Compared with the control group, the difference was significant (P< 0.05); Immunohistochemistry showed that the relative contents of FN and TGF-β_2 in the group with chronic asthma was significantly lower than that in the control group, and there was also a significant difference between the two groups (P<0.05). Conclusion: The slow-reducing treatment can reduce the accumulation of extracellular matrix component FN in the glomerulus by inhibiting the excessive TGF-β 2 level, delay the progression of renal failure, and protect the residual renal function.