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目的探讨参麦注射液对脂多糖(LPS)诱导的A549细胞损伤的保护作用。方法培养A549细胞分为三组:C组为空白对照;LPS组加入LPS 10μg/ml诱导;SM组为LPS诱导加5‰参麦注射液处理24h和48h。qRT-PCR检测炎症因子和凋亡相关基因表达水平,流式细胞术检测细胞凋亡率。结果 SM组及C组细胞凋亡率均低于LPS组[(7.25±0.17)%和(7.63±0.52)%vs.(11.7±0.48)%](P<0.05)。SM组及C组IL-6和TNF-αmRNA表达均低于LPS组(P<0.05)。与LPS组相比,SM组及C组Bax mRNA水平降低,而Bcl-2mRNA水平升高(P<0.05)。结论参麦注射液通过降低炎症因子水平及上调抑凋亡基因和下调促凋亡基因表达以减轻LPS诱导A549细胞损伤。
Objective To investigate the protective effect of Shenmai injection on lipopolysaccharide (LPS) -induced injury of A549 cells. Methods A549 cells were divided into three groups: control group C was blank control; LPS group was induced by LPS 10μg / ml; SM group was induced by LPS plus 5 ‰ Shenmai injection for 24h and 48h. The levels of inflammatory cytokines and apoptosis related genes were detected by qRT-PCR, and the apoptosis rate was detected by flow cytometry. Results The apoptosis rates of SM group and C group were lower than those of LPS group [(7.25 ± 0.17)% vs (7.63 ± 0.52)% vs (11.7 ± 0.48)%] (P <0.05). The expressions of IL-6 and TNF-α mRNA in SM group and C group were lower than those in LPS group (P <0.05). Compared with LPS group, Bax mRNA level and Bcl-2 mRNA level in SM group and C group increased (P <0.05). Conclusion Shenmai injection can reduce the injury of LPS-induced A549 cells by decreasing the level of inflammatory cytokines and up-regulating the expression of anti-apoptotic genes and down-regulating the expression of pro-apoptotic genes.