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目的建立同时测定黄连药材中小檗碱、巴马汀和药根碱含量的毛细管电泳方法。方法采用聚凝胺-聚丙乙烯磺酸钠涂层毛细管柱(33 cm×50μm I.D.,有效长度为24.7 cm),运行缓冲液为500 mmol.L-1Tris-甲醇(体积比为70∶30)缓冲溶液(8 mol.L-1,磷酸调节pH 7.5),分离电压为22 kV,检测波长为230 nm,温度为20℃,压力自动进样(5 000 Pa×6 s),阴极检测。结果在所选电泳条件下,黄连中的生物碱组分在4.5 m in内得到良好分离,盐酸小檗碱、盐酸巴马汀和盐酸药根碱的线性范围分别为199.8~1 998、48.45~484.5和47.3~473 mg.L-1,线性关系良好,加样回收率分别为102.8%、100.2%和97.7%。结论该方法可应用于黄连药材中生物碱的分析测定。
Objective To establish a capillary electrophoresis method for simultaneous determination of berberine, palmatine and jatrorrhizine in Coptis chinensis. Methods Polybrene - sodium polyacrylate coated capillary column (33 cm × 50 μm ID, effective length of 24.7 cm) was used. Buffer was 500 mmol·L-1 Tris-methanol (70:30 by volume) (8 mol·L-1, pH 7.5). The separation voltage was 22 kV, the detection wavelength was 230 nm, the temperature was 20 ℃ and the autoclave pressure was 5 000 Pa × 6 s. Results Under the selected electrophoresis conditions, the alkaloid components of Coptis chinensis Franch were separated well within 4.5 mins. The linear ranges of berberine hydrochloride, palmatine hydrochloride and jatrorrhizine hydrochloride were 199.8-1988.48 ~ 484.5 and 47.3 ~ 473 mg.L-1, respectively. The recoveries were 102.8%, 100.2% and 97.7%, respectively. Conclusion The method can be applied to the determination of alkaloids in Coptis.