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目的阐述引起广西2008年手足口病流行的肠道病毒71型VP1区序列特征及种系进化分析。方法对从广西2008年HFMD患者分离到的EV71毒株,采用逆转录-聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR),进行VP1编码区序列扩增,并对扩增产物进行序列测定和分析。结果从广西2008年HFMD患者标本中共分离到12株EV71毒株,12株毒株VP1编码区核苷酸长度均为891 bp,在进化树中与C4亚型同处一簇,属于C4亚型C4b分支;各株核苷酸和氨基酸同源性分别为96%~100%和99%~100%,至少存在5条传播链。结论广西2008年EV71流行株属于C4基因亚型C4b分支,与我国近年的EV71流行株具有较高的同源性和共进化性;应加强广西EV71病原监测和研究,掌握EV71流行株的基因型别和特征。
Objective To describe the sequence and phylogenetic analysis of the VP1 region of enterovirus 71 that caused the HFMD epidemic in Guangxi in 2008. Methods EV71 strains isolated from patients with HFMD in Guangxi in 2008 were amplified by VP1 coding region by reverse transcription polymerase chain reaction (RT-PCR), and the amplified products were sequenced Determination and analysis. Results A total of 12 EV71 strains were isolated from specimens of HFMD patients in Guangxi in 2008. The 12 nucleotide sequences of the 12 strains of VP1 were all 891 bp in length and co-located with the C4 subtype in the phylogenetic tree, belonging to the C4 subtype C4b branch. The nucleotide and amino acid homology of each strain was 96% -100% and 99% -100%, respectively. There were at least 5 transmission chains. Conclusion The EV71 strains of Guangxi in 2008 belonged to the C4b subfamily of C4 gene and had high homology and co-evolution with the EV71 epidemic strains in recent years in our country. The EV71 pathogen surveillance and study in Guangxi should be strengthened and the genotypes of EV71 strains Do not and features.