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以除草剂苄嘧磺隆(BSM)为模板,在金电极表面电化学聚合邻氨基苯酚(oAP)制备BSM分子印迹膜传感器。采用循环伏安法和交流阻抗技术对传感器制备过程进行表征,用紫外光谱法研究功能单体oAP和模板分子BSM间的相互作用。以K3Fe(CN)6为电化学探针,利用差分脉冲法研究了印迹膜传感器的响应性能。结果表明:V(乙腈):V(水)=1:2溶液对模板分子BSM的洗脱效果最佳,K3Fe(CN)6的峰电流值与BSM浓度在2.5~180μmol/L范围内呈现较好的线性关系(R2=0.997),检出限为0.8μmol/L。印迹膜传感器达到稳定电流响应时间约为5 min,对BSM具有较好的选择性和稳定性,能够在与BSM结构相似及常见共存物除草剂吡嘧磺隆(PSE)和特丁津(TB)的存在下选择性识别BSM。将该印迹膜传感器用于环境水样加标回收测试,回收率在95.1%~100.5%之间。
The BSM molecularly imprinted membrane sensor was electrochemically polymerized o-aminophenol (oAP) on the surface of gold electrode with the herbicidal bensulfuron-methyl (BSM) as a template. Cyclic voltammetry and AC impedance techniques were used to characterize the sensor preparation process. The interaction between the functional monomer oAP and the template molecule BSM was studied by UV spectroscopy. Using K3Fe (CN) 6 as an electrochemical probe, the differential pulse method was used to study the response of the imprinted membrane sensor. The results showed that the elution of template molecule BSM was best with V (acetonitrile): V (water) = 1: 2 solution. The peak current value of K3Fe (CN) 6 and BSM concentration in the range of 2.5 ~ 180μmol / Good linear relationship (R2 = 0.997), the detection limit of 0.8μmol / L. The imprinted membrane sensor achieves a steady current response time of about 5 min and has good selectivity and stability to BSM. The imprinted membrane sensor can be used in the field of biomaterials with similar structure to BSM and the common coexisting herbicides pyrazosulfuron-methyl (PSE) and terbuthyrin (TB) ) Selectively recognizes BSM. The imprinted membrane sensor was used for environmental water sample spike recovery test, the recovery rate was between 95.1% ~ 100.5%.