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目的考察microRNA-141(miR-141)对人头颈部鳞癌细胞增殖的影响。方法通过qRT-PCR分析19例头颈部鳞状细胞癌患者的肿瘤组织与癌旁正常组织标本中miR-141的表达。通过miR-141mimics或miR-141反义寡核苷酸(ASO)上调或抑制人喉癌细胞株(Hep-2)和人舌鳞状细胞癌细胞株(SCC-9)细胞中miR-141的表达水平。采用MTT实验分析上调或抑制miR-141的表达水平对Hep-2和SCC-9细胞增殖的影响。利用生物信息学方法预测miR-141的靶基因。结果 qRT-PCR的结果表明,头颈部鳞状细胞癌组织的miR-141的表达水平低于癌旁正常组织(P<0.05);上调miR-141的表达能够抑制Hep-2和SCC-9细胞的增殖(P<0.05),而抑制miR-141表达可以促进Hep-2和SCC-9细胞的增殖(P<0.05)。生物信息学方法预测miR-141的靶基因可能为ZEB1基因。结论 miR-141可能通过ZEB1基因抑制头颈部鳞状细胞癌的增殖。
Objective To investigate the effect of microRNA-141 (miR-141) on the proliferation of human head and neck squamous cell carcinoma. Methods qRT-PCR was used to analyze the expression of miR-141 in 19 cases of squamous cell carcinoma of the head and neck with tumor tissue and adjacent normal tissues. MiR-141mimics or miR-141 antisense oligonucleotides (ASO) upregulate or inhibit the expression of miR-141 in human laryngeal squamous cell carcinoma (Hep-2) and human tongue squamous cell carcinoma (SCC-9) The expression level. MTT assay was used to analyze the effect of up-regulated or down-regulated miR-141 expression on the proliferation of Hep-2 and SCC-9 cells. Using bioinformatics method to predict the target gene of miR-141. Results The results of qRT-PCR showed that the expression of miR-141 in head and neck squamous cell carcinoma tissue was lower than that in adjacent normal tissues (P <0.05). Upregulation of miR-141 expression could inhibit the expression of miR-141 in Hep-2 and SCC-9 (P <0.05). Inhibition of miR-141 expression could promote the proliferation of Hep-2 and SCC-9 cells (P <0.05). Bioinformatics prediction of miR-141 target gene may be ZEB1 gene. Conclusion miR-141 may inhibit the proliferation of head and neck squamous cell carcinoma through ZEB1 gene.