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目的将胎鼠的神经干细胞移植到脊髓栓系综合征大鼠的病变脊髓中,观察治疗效果。方法从孕17d大鼠胚胎脊髓中分离、培养神经干细胞并诱导分化,通过免疫组化技术研究证实其特性。制作大鼠脊髓栓系模型,3d后将未分化的神经干细胞注入病变脊髓内,2个月后观察大鼠的运动功能,处死大鼠,研究移植后的干细胞在体内存活、迁移及分化情况。结果实验中分离、培养的神经干细胞能够被诱导分化成神经元、星形胶质细胞和少突胶质细胞。脊髓栓系大鼠移植干细胞后运动功能改善,神经元数目增多,显著好于未移植组。免疫组化方法证实移植后的干细胞在体内可以存活、迁移、分化成神经细胞。结论大鼠胚胎神经干细胞体内、体外均具有多向分化潜能,移植神经干细胞是治疗脊髓栓系神经变性的一种有效途径。
Objective To transplanate fetal rat neural stem cells into the spinal cord of rats with tethered cord syndrome and to observe the therapeutic effect. Methods Neural stem cells were isolated and cultured from embryonic spinal cord of pregnant rats for 17 days and induced to differentiate. Their characteristics were confirmed by immunohistochemistry. After 3 days, the undifferentiated neural stem cells were injected into the diseased spinal cord. Motor function of the rats was observed after 2 months. The rats were killed to study the survival, migration and differentiation of the stem cells after transplantation. Results In experiment, neural stem cells isolated and cultured can be induced to differentiate into neurons, astrocytes and oligodendrocytes. After transplanted stem cells of spinal cord tethered rats improved motor function, the number of neurons increased significantly better than the non-transplanted group. Immunohistochemistry confirmed that the stem cells after transplantation can survive, migrate and differentiate into nerve cells in vivo. Conclusion Rat embryonic neural stem cells have multidirectional differentiation potential both in vitro and in vivo. Transplanted NSCs are an effective way to treat neurodegeneration of tethered cord.