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使用转导有单纯疱疹Ⅰ型病毒胸苷激酶（ＨＳＶ１－ＴＫ）基因的人肝癌ＳＭＭＣ－７７２１细胞株建立了裸鼠移植瘤模型，并进行了在体和体外试验性无环鸟苷（ＡＣＶ）治疗。结果发现基因转导后的肝癌细胞表现出对ＡＣＶ的敏感性，肝癌细胞生长抑制与ＡＣＶ的剂量有关；用药组裸鼠除平均瘤重明显低于对照组外，还可见细胞核分裂指数降低，伴有细胞变性和坏死。结果表明：ＨＳＶ１－ＴＫ基因经逆转录病毒转导后，用ＡＣＶ诱导表达明显抑制了人肝癌细胞的生长。
A nude mouse xenograft tumor model was established using a human hepatoma SMMC-7721 cell line transduced with the herpes simplex virus type I thymidine kinase (HSV1-TK) gene, and in vivo and in vitro experimental acyclovir (ACV) were performed. treatment. The results showed that the gene transduced hepatoma cells showed sensitivity to ACV, and the growth inhibition of hepatoma cells was related to the dose of ACV; in the drug-treated group, the average tumor weight was significantly lower in the nude mice than in the control group, and the mitotic index was also decreased. Cell degeneration and necrosis. The results showed that: HSV1-TK gene was transduced by retrovirus, and the expression of HSV1-TK significantly inhibited the growth of human hepatoma cells.