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采用二乙烯基砜醇、乙二胺和戊二醛活化硝酸纤维素膜，在膜上形成一“结合臂”，该臂含游离的醛基，后者与短肽的游离氨基以肽键结合。以此为基础建立的点免疫结合试验，能区分只有一个残基不同的一组七肽，敏感度达ｐｇ水平。
Activation of the nitrocellulose membrane with divinyl sulfone alcohol, ethylene diamine and glutaraldehyde forms a “binding arm” on the membrane containing free aldehyde groups which are peptide-bonded to the free amino groups of the short peptides . Based on this, the dot immuno-binding assay can distinguish a group of heptapeptides that have only one residue and have a sensitivity of pg.