The ~(99m)Tc-3PRGD_2 targeted SPECT/CT scanning was of significance in detecting differentiated glioma.In this work,the diagnostic value of ~(99m)Tc-3PRGD_2 scintigraphy in brain glioma was evaluated by the ten clinically verified brain glioma patients after obtaining informed consent.The patients first accepted X-ray imaging to localize the detecting regions before administrating with ~(99m)Tc-3PRGD_2 at a mean radioactivity of 849±115 MBq via single intravenous bolus injection 2 h prior to SPECT/CT imaging.Tumor samples for detectingαvβ3 were collected by surgical operations two weeks after the scintigraphy.The results of CT and SPECT scanning were merged and compared.The correlation between tumor occupation(T/N ratio) andαvβ3 expression level were analyzed.The T/N ratios in brain glioma were proportionally correlated to av(33 positive cell percentage(R~2=0.9253,p<0.05).This study primarily evaluated the clinical application of ~(99m)Tc-3PRGD_2 SPECT scintigraphy on brain glioma.The more pathological types and detecting strategies covering a large amount of samples would aid to clarify the potentials. The ~ (99m) Tc-3PRGD_2 targeted SPECT / CT scanning was of significance in detecting differentiated glioma. This work, the diagnostic value of ~ (99m) Tc-3PRGD_2 scintigraphy in brain glioma was evaluated by the ten clinically verified brain glioma patients after obtaining informed consent. The patient first accepted X-ray imaging to localize the detecting regions before administrating with ~ (99m) Tc-3PRGD_2 at a mean radioactivity of 849 ± 115 MBq via single intravenous bolus injection 2 h prior to SPECT / CT imaging . Tumor samples for detecting αvβ3 were collected by surgical operations two weeks after the scintigraphy. The results of CT and SPECT scanning were merged and compared. The correlation between tumor occupation (T / N ratio) and ανβ3 expression level were analyzed. The T / N ratios in brain glioma were proportionally correlated to av (33 positive cell percentage (R ~ 2 = 0.9253, p <0.05). This study is evaluated as the clinical application of 99m Tc-3PRGD_2 SPECT scintigraphy on brain glioma The more pathological types and detecting strategies covering a large amount of samples would aid to clarify the potentials.