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目的:观察奥美沙坦对大鼠移植静脉内膜重构过程中整合素β-3(integrinβ-3)黏着斑激酶(focal adhesion kinase,FAK)表达的影响。方法:建立大鼠移植静脉桥模型,将36只雄性SD大鼠被随机分成模型对照组、奥美沙坦组及生理盐水对照组,每组12只。取移植静脉切片、HE染色观察各组血管壁内膜的厚度。采用免疫组化染色法观察各组血管平滑肌细胞(SMC)增殖核抗原(PCNA)的表达。采用Western blot法检测整合素β-3和FAK的表达。结果:与模型对照组相比,奥美沙坦组内膜的厚度明显减轻(P<0.05);PCNA和FAK的表达明显降低(P<0.05)。生理盐水对照组与模型对照组相比,内膜的厚度无明显减轻;PCNA和FAK的表达无明显改变。结论:大鼠移植静脉内膜重构过程中,伴随整合素β-3/FAK信号蛋白的表达,奥美沙坦可抑制大鼠移植静脉内膜重构,此作用可能与下调FAK信号蛋白的表达有关。
OBJECTIVE: To observe the effects of olmesartan on the expression of focal adhesion kinase (FAK) in rat integrin β-3 induced by intimal remodeling. Methods: The rat vein graft model was established. Thirty-six male SD rats were randomly divided into model control group, olmesartan group and saline control group, with 12 rats in each group. Take the vein graft sections, HE staining was used to observe the thickness of the vascular wall intima. Immunohistochemical staining was used to observe the expression of proliferating cell nuclear antigen (PCNA) in vascular smooth muscle cells (SMCs). Western blot was used to detect the expression of integrin β-3 and FAK. Results: Compared with the model control group, the thickness of the intima of olmesartan group was significantly reduced (P <0.05), and the expression of PCNA and FAK was significantly decreased (P <0.05). Compared with the model control group, the thickness of intima in the saline control group was not significantly reduced; the expression of PCNA and FAK had no significant change. CONCLUSION: Olmesartan can inhibit the intimal remodeling in rat vein graft with the expression of integrin β-3 / FAK signal during intimal remodeling, which may be related to the downregulation of FAK signaling protein related.