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目的建立同时测定婴幼儿配方乳粉中叶黄素和β-胡萝卜素的快速测定方法。方法奶粉样品加2mL 50%氢氧化钾碱溶液,在70℃水浴下皂化15min,经石油醚:乙醚(1:1,v/v)提取,水洗、浓缩后,C30色谱柱分离,在450 nm波长处检测,外标法定量。结果叶黄素和β-胡萝卜素在0.2~4.0μg/mL范围内线性良好,相关系数:R2>0.9990;三浓度水平加标回收率均在80%~90%之间,样品及三个浓度水平加标6次重复试验RSD均<5%。结论该方法准确、简单、快速且能同时测定婴儿配方乳粉中的叶黄素和β-胡萝卜素含量。
Objective To establish a rapid method for the simultaneous determination of lutein and β-carotene in infant formula milk powder. Methods The milk powder sample was added with 2mL 50% potassium hydroxide alkali solution, saponified in water bath at 70 ℃ for 15min, extracted with petroleum ether: ether (1: 1, v / v), washed with water and concentrated. Wavelength detection, external standard method. Results Lutein and β-carotene showed a good linearity in the range of 0.2-4.0 μg / mL, with a correlation coefficient of R2> 0.9990. The spiked recoveries of three concentrations ranged from 80% to 90%. The concentrations of three concentrations The standard RSD of 6 replicates was less than 5%. Conclusion The method is accurate, simple, rapid and capable of simultaneous determination of lutein and β-carotene in infant formula milk powder.