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目的 寻找抗肠道病毒 (EV)感染的安全有效的药物。方法 采用细胞形态观察、MTT法比色检测利巴韦林、双黄连、大蒜素的细胞毒性 ,并以观察CPE、MTT法比色及蚀斑抑制实验判断其抗CBV3和ECHO11的活性和进行三者间及后二者病毒吸附前后的药效比较。结果 ①利巴韦林TC5 0为 2mg ml,在 1mg ml～ 1 5mg ml时有抑制CBV3和ECHO11的活性。②双黄连TC5 0为 5mg ml,在 0 5mg ml时即有抑制CBV3和ECHO11的活性 ,且与药物浓度呈正相关。③大蒜素TC5 0为12 5 μg ml,在 2 5 μg ml～ 7 5 μg ml时有抑制CBV3和ECHO11的活性。④ 1 5mg ml利巴韦林对CBV3及ECHO11的蚀斑抑制率分别为 43 2 %和 37 2 % ,2 5mg ml双黄连为 81 1%和 88 0 % ,5 μg ml大蒜素为 6 6 2 %和 77 4%。⑤双黄连在病毒吸附前用药蚀斑抑制率高于吸附后用药 (P <0 0 5 ) ;大蒜素的差异则不显著 (P >0 0 5 )。结论 三种药物均有体外抗CBV3、ECHO11的作用 ,但双黄连、大蒜素优于利巴韦林。三种药物中以双黄连毒性最小 ,抑制病毒活性最高。双黄连在病毒吸附前用药抗病毒活性优于病毒吸附后 ,有一定预防作用。
Aim To find a safe and effective anti-enterovirus (EV) infection. Methods Cytotoxicity of ribavirin, shuanghuanglian and allicin was detected by MTT colorimetric method. The activity of CBV3 and ECHO11 against CBV3 and ECHO11 was evaluated by MTT colorimetric and plaque inhibition assay. Comparison of the efficacy of the two before and after virus adsorption. Results ① Ribavirin TC5 0 was 2 mg ml, which inhibited the activity of CBV3 and ECHO11 at 1 mg ml ~ 15 mg ml. ② Shuanghuanglian TC5 0 as 5mg ml, at 0 5mg ml that inhibit the activity of CBV3 and ECHO11, and the drug concentration was positively correlated. ③ Allicin TC50 is 125 μg ml, which inhibits the activity of CBV3 and ECHO11 when the concentration is 25 μg ml-7 5 μg ml. ④ The plaque inhibition rates of CBV3 and ECHO11 with 1 5 mg ml ribavirin were 43 2% and 37 2%, respectively. The 25 mg ml Shuanghuang was 81 1% and 88 0%, while the 5 μg ml allicin was 6 6 2 % And 77 4%. ⑤ Shuanghuanglian in the virus before the adsorption of plaque inhibition rate was higher than that after adsorption (P <0 05); allicin was not significantly different (P> 0.05). Conclusion All three drugs have anti-CBV3 and ECHO11 effects in vitro, but Shuanghuanglian and Allicin are better than ribavirin. The three drugs to minimize the Shuanghuanglv, inhibit the highest virus activity. Shuanghuanglian before the virus adsorption drug anti-viral activity better than the virus adsorption, there is a preventive effect.