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成纤维细胞生长因子(Fibroblast Growth Factor筒称FGF)广泛存在于多种组织之中,包括脑、垂体、视网膜、软骨、肾上腺等,甚至也存在于肿瘤如软骨肉瘤之中。这类生长因子与肝素具有很高的亲合力因而为其提纯、分离提供了极为有利的条件。经SDS-聚丙烯酰胺,等电点聚焦电泳以及对肝素的亲合性的研究表明:FGF有两种基本结构,一为酸性成纤维细胞生长因子(简称酸性FGF);另一为碱性成纤维细胞生长因子(简称碱性FGF)。经体外系统检测证实:二者均可刺激中胚叶来源的成纤维细胞或内皮细胞的增殖,尤其值得注意的是碱性FGF还是有效的血管生成剂。
Fibroblast growth factor (FGF) is widely found in many tissues, including the brain, pituitary, retina, cartilage, adrenal glands, and even in tumors such as chondrosarcoma. These kinds of growth factors have high affinity with heparin and thus provide extremely favorable conditions for their purification and separation. SDS-polyacrylamide, isoelectric focusing electrophoresis and heparin affinity study showed that: FGF has two basic structures, one for acidic fibroblast growth factor (acidic FGF); the other is alkaline into Fibroblast growth factor (referred to as basic FGF). It has been confirmed by in vitro system that both of them can stimulate the proliferation of mesodermal derived fibroblasts or endothelial cells, and it is especially notable that basic FGF is also an effective angiogenesis agent.