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目的探讨围生期暴露双酚A(bispheno1 A,BPA)对成年期雌性子代大鼠血脂及激素敏感性脂肪酶(hormonesensitive lipase,HSL)mRNA、蛋白表达的影响。方法将21只清洁级SD妊娠大鼠分为3组,分别为对照(含1%无水乙醇的水)组和低(1μg/ml)、高(10μg/ml)剂量BPA暴露组,每组7只。从妊娠第6天至哺乳期(出生后20 d),母鼠采用自由饮水方式染毒;断乳后,雌性仔鼠采用自由饮水(不含BPA)方式继续喂养80 d。测定雌性仔鼠体重、空腹血脂[包括血清甘油三酯(triglycerides,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)和低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)],并检测内脏脂肪组织HSL mRNA及蛋白的表达水平。结果与对照组比较,各剂量BPA染毒组子代雌鼠体重显著增高,各剂量BPA暴露组雌性仔鼠血清中HDL-C水平降低,仅高剂量BPA暴露组血清中TG水平增高,差异均有统计学意义(P<0.01);而各剂量BPA暴露组雌性仔鼠血清中TC和LDL-C水平均无明显改变。与对照组比较,各剂量BPA暴露组雌性仔鼠脂肪组织中的HSL mRNA和蛋白表达水平均降低,差异有统计学意义(P<0.05);且随着BPA暴露剂量的升高,雌性仔鼠脂肪组织中的HSL mRNA和蛋白表达水平均呈下降趋势。结论发育早期暴露BPA可引起成年期子代雌鼠体重增加和血脂代谢紊乱,这可能与其下调内脏脂肪组织HSL表达有关。
Objective To investigate the effect of bispheno1 A (BPA) exposure on blood lipids and hormone-sensitive lipase (HSL) mRNA and protein expression in adult offspring rats. Methods Twenty-one clean SD pregnant rats were divided into three groups: control (water with 1% absolute ethanol) and low (1μg / ml) and high (10μg / ml) 7 only From 6th day of pregnancy to lactation (20 days after birth), female rats were exposed to drinking water freely. After weaning, female offspring were fed with free water (without BPA) for 80 days. The body weight, fasting blood lipid [including triglycerides (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (low density lipoprotein cholesterol, LDL-C)], and detect the expression of HSL mRNA and protein in visceral adipose tissue. Results Compared with the control group, the body weight of the offspring of each dose of BPA significantly increased the HDL-C levels in serum of female offspring in BPA exposed groups, and increased in serum of high dose BPA exposed groups (P <0.01). However, the levels of TC and LDL-C in serum of female offspring in each dose of BPA exposed group had no obvious changes. Compared with the control group, HSL mRNA and protein expression in adipose tissue of the offspring exposed to each dose of BPA decreased significantly (P <0.05). With the increase of BPA exposure, the female offspring Adipose tissue HSL mRNA and protein expression levels showed a downward trend. Conclusion Exposure to BPA during early development may cause weight gain and dyslipidemia in female offsprings in adolescence, which may be related to down-regulation of HSL expression in visceral adipose tissue.