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目的观察参黄冲剂对衰老患者P16基因mRNA定量表达、血清一氧化氮(NO)和超氧化物歧化酶(SOD)含量的影响,探讨其延缓衰老作用机制。方法将60例衰老患者随机分为参黄冲剂组和对照组,每组30例。在常规疾病治疗基础上,参黄冲剂组加用参黄冲剂,对照组加用甲磺酸双氢麦角毒碱片,均连续服用8周。观察治疗前后患者临床症状及P16基因mRNA定量表达、血清NO和SOD含量变化。结果治疗后参黄冲剂组P16基因mRNA扩增曲线起始循环数明显变大(P<0.05),P16基因mRNA定量表达与对照组比较差异有统计学意义(P<0.05)。参黄冲剂组治疗后血清NO含量和SOD水平明显升高(P<0.05),且明显高于对照组(P<0.05)。参黄冲剂组总有效率为80.0%(24/30),对照组为43.3%(13/30),差异有统计学意义(P<0.01)。结论参黄冲剂可有效改善衰老患者的临床症状,提高日常生活能力,提高血清NO含量和SOD活性,减少P16基因mRNA的表达,其机制可能是通过抑制衰老细胞的增殖而起到延缓衰老的作用。
Objective To observe the effects of Shenhuang granule on the quantitative expression of P16 mRNA, serum nitric oxide (NO) and superoxide dismutase (SOD) in senescent patients, and to explore its anti-aging mechanism. Methods Sixty elderly patients were randomly divided into Shenhuang granule group and control group, with 30 cases in each group. On the basis of conventional disease treatment, Shenhuang granule plus Shenhuang granule and control group plus dihydroergotoxine mesylate tablets were given for 8 weeks continuously. Clinical symptoms and P16 mRNA expression were observed before and after treatment, and the changes of serum NO and SOD were observed. Results After treatment, the initial cycle number of P16 gene mRNA in SHR group significantly increased (P <0.05). The quantitative expression of P16 gene mRNA had statistical significance compared with the control group (P <0.05). After treatment with Shenhuang granule, serum NO and SOD levels were significantly increased (P <0.05), and were significantly higher than those in the control group (P <0.05). The total effective rate was 80.0% (24/30) in SHR group and 43.3% (13/30) in control group, the difference was statistically significant (P <0.01). Conclusion Shenhuang granule can effectively improve the clinical symptoms, improve daily living ability, increase serum NO level and SOD activity and decrease P16 mRNA expression, which may be the mechanism of anti-aging by inhibiting the proliferation of senescent cells .