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目的:建立一种测定重楼皂苷Ⅰ,Ⅱ的含量测定方法,用于比较重楼乙醇提物与重楼配方颗粒中皂苷的含量。方法:采用C18色谱柱,以乙腈-水(42∶58)为流动相,检测波长210 nm,流速1 mL.min-1,柱温室温。结果:在0.043 5～0.870 0g.L-1,重楼皂苷Ⅰ的峰面积与浓度有良好的线性,r=0.999 1;在0.038～0.760 0 g.L-1,重楼皂苷Ⅱ的峰面积与浓度有良好的线性,r=0.999 7,重楼皂苷Ⅰ,Ⅱ的平均回收率为99.90%,100.2%,RSD分别为1.7%,1.7%。醇提物与配方颗粒中皂苷Ⅰ,Ⅱ的总含量分别为34.7%,3.1%。结论:该法操作简单,出峰时间短,两峰分离度好,测得的醇提物中皂苷Ⅰ,Ⅱ含量明显高于重楼配方颗粒。
OBJECTIVE: To establish a method for the determination of polysaccharide Ⅰ, Ⅱ in Rhizoma Paridis, which is used to compare the content of saponins in ethanol extract of Chonglou Tower and the formula granules of Chonglou. Methods: The mobile phase was acetonitrile-water (42:58). The detection wavelength was 210 nm and the flow rate was 1 mL.min-1 on a C18 column at room temperature. Results: In the range of 0.043 5 ~ 0.870 0g.L-1, the peak area and concentration of SaponinⅠwere linear with r = 0.999 1. The peak areas and concentrations of Saponins Ⅱ at 0.038 ~ 0.760 gL- The average recoveries of polysaccharides Ⅰ and Ⅱ were 99.90% and 100.2%, respectively. The RSDs were 1.7% and 1.7%, respectively. The total contents of saponins I and II in alcohol extract and formula granule were 34.7% and 3.1% respectively. Conclusion: The method is simple, the peak time is short, the peak separation is good, and the content of saponins Ⅰ and Ⅱ in the alcohol extract is obviously higher than that of Zhonglou formula particles.