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目的研究我所发现的丙型肝炎病毒(HCV)1b基因型5′非编码区(5′NCR)222位C-T变异的病毒株,是否为1型中的其他亚型。方法对64例HCV1b型的样本进行5′NCR扩增后作BamHI酶切,并从有此BamHⅠ位点的样品中随机选取了6例样品扩增5′NCR和NS5B区,测序后对5′NCR进行序列分析,NS5B区序列分别与GenBank中1型的各亚型序列以及38株来自世界不同地区的1b型参考序列构建遗传进化树。结果6例样本与GenBank中1型的各亚型NS5B区遗传进化树分析结果显示这6株都属于1b型,而非1型的其他亚型,6株变异株与38株来自世界不同地区的1b型参考序列NS5B区的进化树分析结果显示,6例样品并未形成1b型中的独立分支,在遗传进化上与其他38株HCV1b型病毒株没有明显差异。结论本文的研究证实了我们所发现的HCV1b型5′非编码区有BamHⅠ位点的病毒株确为1b型的变异株,并非1型的其他亚型。而这种变异与干扰素耐药是否具有相关性,尚有待于进一步研究。
Objective To investigate whether the C strain variant at position 222 of the 5 ’non-coding region (5’NCR) of the HCV genotype 1b in HCV I was found to be other subtypes of type 1. Methods Sixty-four samples of HCV type 1b were amplified by 5 ’NCR and then digested with BamHI. Six samples were randomly selected from the samples with this BamHⅠ site to amplify 5’NCR and NS5B. After sequencing, NCR for sequence analysis. The NS5B region sequence was used to construct the phylogenetic tree with each type 1 subtype in GenBank and 38 type 1b reference sequences from different regions of the world. Results The phylogenetic tree analysis of 6 NS5B subtypes in 6 samples and GenBank 1 revealed that the 6 isolates belonged to type 1b rather than other subtypes of type 1 and 6 to 38 isolates from different regions of the world The results of phylogenetic tree analysis showed that 6 samples did not form an independent branch in type 1b, and there was no significant difference in genetic evolution between the other 38 strains of HCV1b. Conclusions Our study confirms that the type of strain in which the BamH I site in the 5 ’non-coding region of HCV 1b genotype was found to be a type 1b mutant was not the other subtype of type 1. Whether this variation is related to interferon resistance remains to be further studied.