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目的 研究上皮性卵巢癌细胞凋亡、增殖状态和肿瘤微血管生成及其与肿瘤复发之间的关系。方法 对 1996年 10月至 1999年 10月间 45例上皮性卵巢癌石蜡组织切片采用DNA原位缺口末端标记 (TUNEL)方法,检测细胞凋亡指数(AI);应用FⅧ相关抗原及增殖细胞核抗原 (PCNA),采用SABC法,检测卵巢癌中的肿瘤微血管密度(MVD)及细胞增殖状态(PI)。结果 不同的组织类别,临床分期,组织分级中AI、PI差异无显著性(P>0.05)。卵巢癌中的AI/PI平均值为 0.0621±0.0281,临床Ⅲ ~Ⅳ期肿瘤AI/PI显著低于临床Ⅰ ~Ⅱ期肿瘤(P<0.05);MVD在不同组织类别,不同临床分期中的差异有显著性意义(P<0.05); 6例复发性上皮性卵巢癌中AI/PI与MVD均高于未复发者。结论 上皮性卵巢癌组织中凋亡的发生远低于细胞的增殖,细胞凋亡的相对减少与细胞增殖共同参与了肿瘤的发展。随着卵巢癌的进展,肿瘤血管生成是增多的。复发性上皮性卵巢癌中AI/PI与MVD均高于未复发者。
Objective To study the relationship between epithelial ovarian cancer cell apoptosis, proliferation status, tumor angiogenesis and tumor recurrence. Methods Totally 45 cases of epithelial ovarian cancer paraffin sections from October 1996 to October 1999 were tested for apoptosis index (AI) by DNA nick end labeling (TUNEL) method. FⅧ-associated antigen and proliferating cell nuclear antigen (PCNA). SABC method was used to detect tumor microvessel density (MVD) and cell proliferation status (PI) in ovarian cancer. Results There was no significant difference in AI and PI between different histological categories, clinical stage and histological grade (P> 0.05). The AI / PI in ovarian cancer was 0.0621 ± 0.0281, and the AI / PI in clinical stage Ⅲ ~ Ⅳ was significantly lower than that in clinical stage Ⅰ ~ Ⅱ (P <0.05). The difference of MVD in different histological types and clinical stages (P <0.05). AI / PI and MVD in 6 cases of recurrent epithelial ovarian cancer were significantly higher than those of non-recurrent ones. Conclusion The incidence of apoptosis in epithelial ovarian cancer is much lower than that of the cell proliferation. The relative reduction of apoptosis and cell proliferation are involved in the development of the tumor. As ovarian cancer progresses, tumor angiogenesis is increased. AI / PI and MVD in recurrent epithelial ovarian cancer were higher than those without recurrence.