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分别用灿烂弧菌(Vibrio splendidus)和鳗弧菌(V.anguillarum)对仿刺参(Apostichopus japonicus)进行注射感染试验,取注射后0、3、6、9、12、24 h的仿刺参体壁、肠道、呼吸树、触手和体腔细胞5种组织或细胞进行了实时荧光定量PCR检测,分析硫氧还蛋白(Thioredoxin,Trx)基因在这5种组织或细胞中的表达特征,探讨了硫氧还蛋白在仿刺参先天免疫中的作用。结果显示,Trx基因在仿刺参体壁、肠道、触手、呼吸树及体腔细胞中均表达;在灿烂弧菌刺激下各组织或细胞中Trx基因变化趋势相似,呈现一种“突增-下降-再突增”的双峰型模式;鳗弧菌刺激下Trx基因表达量变化同样呈现先升高后降低的趋势,但与灿烂弧菌组相比反应时间与上调幅度都不同。总而言之,Trx基因在仿刺参体内有组成型和诱导型两种表达模式,并存在表达的组织特异性和病原特异性;Trx基因参与了仿刺参对病原感染的免疫应答,使机体免受氧化胁迫,在抵御外界病原入侵、维持胞内氧化还原状态方面起到重要作用。
The infection experiments of Apostichopus japonicus were carried out with Vibrio splendidus and V. anguillarum, respectively. The specimens were taken at 0, 3, 6, 9, 12 and 24 h after injection The expression of Thioredoxin (Trx) gene in these five kinds of tissues or cells was analyzed by real-time fluorescent quantitative polymerase chain reaction (PCR) in five kinds of tissues or cells including the body wall, intestine, respiratory tree, tentacle and body cavity. The role of thioredoxin in the innate immunity of the sea cucumber. The results showed that the Trx gene was expressed in the body wall, intestinal tract, tentacles, respiratory tree and body cavity cells of Apostichopus japonicus. The changes of Trx gene in tissues or cells stimulated by V. splendens were similar, showing a sudden increase - decrease - then suddenly increase "bimodal pattern; Trichoderma anguillarum Trx gene expression changes also showed a trend of first increased and then decreased, but with the splendid group Vibrio reaction time and amplitude are different. In summary, the Trx gene has constitutive and inducible expression patterns in A. glabra and its tissue-specific and pathogen-specific expression. The Trx gene participates in the immune response of S. ginseng to pathogen infection and protects the organism from Oxidative stress plays an important role in resisting external pathogen invasion and maintaining intracellular redox status.