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以亚甲蓝和苯甲酸为羟自由基捕获剂，用分光光度法研究了过亚硝酸根分解反应的机制。研究发现过亚硝酸根在生理ｐＨＴ分解时有羟自由基与亚甲蓝和苯甲酸的加合物产生。亚甲蓝和乙醇不影响过亚硝酸根的分解速率。羟自由基加合物生成速率对过亚硝酸根浓度为一级，对捕获剂浓度接近为零级。表明过亚硝酸根在生理ｐＨ下分解时有羟自由基产生。
Using methylene blue and benzoic acid as hydroxyl radical scavengers, the mechanism of peroxynitrite decomposition was studied by spectrophotometry. Studies have found that nitrite in the physiological pHT decomposition of hydroxyl radicals and methylene blue and benzoic acid adduct production. Methylene blue and ethanol do not affect the nitrite decomposition rate. The rate of formation of hydroxyl radical adduct was one order of magnitude for peroxynitrite and close to zero for capture agent. It shows that when nitrite decomposes at physiological pH, hydroxyl radicals are produced.