论文部分内容阅读
目的探讨2012~2013年杭州及周边地区儿童麻疹病毒(Measles Virus,MV)流行株核蛋白的基因特征。方法用逆转录-聚合酶链反应(RT-PCR)检测麻疹患者的咽拭子标本,对部分PCR扩增阳性的片段进行基因测序、并绘制系统进化树进行同源性分析。结果从杭州市儿童医院2012~2013年80例儿童麻疹患者的咽拭子中检测到29例MV株,经基因测序及系统进化树分析后均为H1a基因型,与该型参考株china93-2﹑china93-4核苷酸同源性为97.7%~98.9%;2012年基因型组内遗传距离(0.007)最小,2012年和2013年基因型之间的组间遗传距离(0.013)最小,而2012年、2013年基因型与A型(疫苗株)的组间遗传距离均较大,分别为0.085、0.087。结论 2012~2013年杭州地区儿童MV的优势亚型为H1a亚型,与中国其他地区报告的流行株一致。2012年与2013年的MV基因比较遗传变异较小,而与疫苗株比较遗传变异较大。
Objective To investigate the genetic characteristics of nucleoprotein of measles virus (MV) in Hangzhou and surrounding areas from 2012 to 2013. Methods The throat swab samples of measles patients were detected by reverse transcription polymerase chain reaction (RT-PCR). Some of the PCR-positive fragments were sequenced and the phylogenetic tree was drawn for homology analysis. Results Thirty-nine MV strains were detected in throat swabs from 80 children with measles in Hangzhou Children’s Hospital from 2012 to 2013. The genotypes were all H1a genotypes after gene sequencing and phylogenetic tree analysis, , The nucleotide homology of china93-4 was 97.7% -98.9%. The genetic distance (0.007) in genotype group in 2012 was the smallest, and the genetic distance (0.013) between genotypes in 2012 and 2013 was the smallest In 2012, the genetic distance between genotypes and type A (vaccine strains) in 2013 was large, which were 0.085 and 0.087, respectively. Conclusion The predominant subtype of MV for children in Hangzhou from 2012 to 2013 was H1a subtype, which is consistent with the reported epidemic strains in other regions of China. Compared with the MV gene in 2012 and 2013, the genetic variation was smaller, but the genetic variation was larger than that in the vaccine strain.