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目的 :探讨榄香烯抑制人喉肿瘤细胞 (HEp 2 )过程中与凋亡相关的Caspase 3活性的改变 ,以及真核细胞翻译起始因子 (eIF)家族成员 (eIF4E、eIF4G等 )和与肿瘤新生血管有关的碱性纤维母细胞生长因子 (bFGF)、血管内皮生长因子 (VEGF)的表达。方法 :使用比色法 ,研究榄香烯诱导人喉鳞状细胞癌细胞株HEp 2细胞凋亡时Caspase 3活性的变化 ;并使用Westernblot和RT PCR法检测榄香烯作用的HEp 2细胞中eIF4E、eIF4G、bFGF、VEGF在mRNA和蛋白水平的表达。结果 :Caspase 3活性在药物作用 12h达到最高 ,并随药物作用时间的延长而下降 ;随着榄香烯作用时间的延长 ,eIF4E、eIF4G、bFGF、VEGF蛋白表达下降 ,bFGF、VEGF的mRNA含量亦下降。结论 :榄香烯抑制HEp 2细胞生长可引起Caspase 3活性的改变 ;eIF4E、eIF4G的表达下降与bFGF、VEGF的表达呈正相关 ;bFGF、VEGF表达的下降与eIF家族成员表达的下降所引起的翻译水平的抑制有关。
OBJECTIVE: To investigate the apoptosis-related changes of Caspase 3 and the expression of eIF4E, eIF4G and so on in eukaryotic cells during the inhibition of elemene in human laryngeal carcinoma cells (HEp 2) Neovascularization-related basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) expression. Methods: The colorimetric method was used to study the changes of Caspase 3 activity in elemene induced apoptosis in human laryngeal squamous cell carcinoma cell line HEp 2. The expression of eIF4E was detected by Western blot and RT-PCR in HEp 2 cells , EIF4G, bFGF, VEGF expression at mRNA and protein levels. Results: The activity of Caspase 3 reached the peak at 12h, and decreased with the prolongation of the drug duration. The expression of eIF4E, eIF4G, bFGF and VEGF decreased and the mRNA content of bFGF and VEGF also decreased with the prolongation of elemene decline. CONCLUSION: Elemene inhibits the growth of HEp 2 cells and induces the alteration of Caspase 3 activity. The decrease of eIF4E and eIF4G is positively correlated with the expression of bFGF and VEGF. The decrease of bFGF and VEGF and the decrease of eIF family member expression The level of inhibition related.