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目的:探讨凝血蛋白酶因子Xa(FXa)的效应蛋白酶受体-1(EPR-1)在鼻咽癌(NPC)组织中的表达及其与细胞增殖、凋亡之间的关系。方法:用免疫组织化学SP法检测NPC组织、癌旁交界组织各42例及鼻咽部黏膜慢性炎症组织30例中EPR-1、增殖细胞核抗原Ki-67的表达,以Ki-67代表细胞增殖指数(PI);用TdT酶介导的生物素化dUTP缺口末端标记技术(TUNEL法)检测凋亡指数(AI)并进行统计学分析。结果:EPR-1在NPC的组织、癌旁交界组织、鼻咽部黏膜慢性炎症组织中的阳性表达率分别为76.1%、90.5%、100.0%,3组间差异有统计学意义(均P<0.01),与AI呈正相关(r=0.551,P<0.01),与PI呈负相关(r=-0.338,P<0.05)。结论:EPR-1促进细胞凋亡,抑制细胞增殖,为以Survivin为靶向的NPC治疗开创新的途径。
Objective: To investigate the expression of the effector protease receptor-1 (EPR-1) of coagulation protease factor Xa (FXa) in nasopharyngeal carcinoma (NPC) and its relationship with cell proliferation and apoptosis. Methods: Immunohistochemical SP method was used to detect the expression of EPR-1, Ki-67 and Ki-67 in 42 cases of NPC, adjacent para-tumor tissues and 30 cases of nasopharyngeal mucosal chronic inflammation tissues respectively. Ki-67 was used to represent the cell proliferation (PI). Apoptotic index (AI) was detected by TdT-mediated biotinylated dUTP nick end labeling (TUNEL) and statistical analysis was performed. Results: The positive expression rate of EPR-1 in NPC tissues, paraneoplastic junctional tissues and nasopharyngeal mucosa chronic inflammatory tissues were 76.1%, 90.5% and 100.0%, respectively, with significant difference (all P < 0.01), and had a positive correlation with AI (r = 0.551, P <0.01), but negatively correlated with PI (r = -0.338, P <0.05). Conclusion: EPR-1 can promote cell apoptosis and inhibit cell proliferation, opening up a new way for Survivin-targeted NPC therapy.