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目的 观察磷脂酶 A2 (phospholipase A2 ,PL A2 )活性变化对高糖或 (和 )高胰岛素 (Ins)环境下培养大鼠血管平滑肌细胞 (vascular smooth muscle cell,VSMC)增殖的影响。方法 在含 2 2m mol· L- 1 Glu或 (和 ) 10 0 Mu· L- 1 Ins的 DMEM培养液中加入 2～ 2 0μmol· L- 1 (终浓度 )的PL A2抑制剂 AACOCF3与 VSMC共同孵育 72 h后 ,用 [3H ]-油酸标记法测定培养上清液中 PL A2活性 ,用细胞计数法和 [3H]-胸腺嘧啶核苷掺入实验观察 VMSC增殖、DNA合成、用流式细胞仪分析 VSMC的细胞周期。结果 AACOCF3浓度为 10～ 2 0 μm ol· L- 1时 ,VSMC的增殖和 DNA合成 ,S期与 G0 +M期细胞百分比 ,抑制剂组均显著低于高糖或 (和 )高 Ins组 (P <0 .0 5 ) ,AACOCF3浓度为 2μm ol· L- 1 时 ,抑制剂组上述指标较高糖或 (和 )高 Ins组下降 ,但差别无显著性 (P >0 .0 5 )。结论 降低 PL A2 活性可抑制高糖或 (和 )高 Ins介导的 VSMC增殖。
Objective To observe the effects of phospholipase A2 (PL A2) activity on the proliferation of vascular smooth muscle cells (VSMC) cultured in high glucose or high insulin (Ins). Methods AACOCF3 with 2 ~ 200μmol · L-1 (final concentration) of PL A2 inhibitor was added to VSMC in DMEM medium containing 2 mol·L-1 Glu or 10 mol·L-1 Ins After incubation for 72 h, the activity of PL A2 in the culture supernatant was determined by [3H] -oleic acid labeling method. The proliferation and DNA synthesis of VMSC were observed by cytometry and [3H] -thymidine incorporation. Cytometry Cell cycle analysis of VSMCs. Results When the concentration of AACOCF3 was 10 ~ 20 μmol · L-1, the proliferation and DNA synthesis of VSMC and the percentage of cells in S and G0 + M phases were significantly lower than those in high glucose or (and) high Ins group P <0.05). When the concentration of AACOCF3 was 2μmol · L-1, the above indexes in inhibitor group were lower than those in high glucose group or (and) high Ins group, but the difference was not significant (P> 0.05). Conclusions Decreasing PL A2 activity can inhibit the proliferation of VSMC induced by high glucose or (and) high Ins.