Large acanthomorphic acritarchs have been found in chert of the Ediacaran Doushantuo Formation at several localities in South China,including the East Yangtze Gorges of Hubei Province, Weng’an area of Guizhou Province,and elsewhere.However,their potential for biostratigraphic subdivision and correlation of Ediacaran successions is limited by facies control,taphonomic biases, and taxonomic problems.In the Yangtze Gorges,the Doushantuo Formation is generally subdivided into four lithologic members.However,in the Weng’an area,the Doushantuo Formation comprises just a lower and upper part separated by a mid-Doushantuo erosional surface.In the Yangtze Gorges at the Zhangcunping section,the Doushantuo succession is similar to that at Weng’an.So far,the correlation between the Yangtze Gorges and Weng’an area,and elsewhere has been an issue of debate.To resolve the debate,we selected eight sections in the Yangtze Gorges area and systemically sampled chert nodules of the Doushantuo Formation,focusing in particular on the upper Doushantuo Formation.Our data confirm two different assemblages appearing separately in the second and third members,which are separated by a negativeδ~(13)C excursion(EN2).The lower assemblage is characterized by Tianzhushania and a diverse suite of large acanthomorphic acritarchs.The upper assemblage is distinguished from the lower assemblage,by(1) absence of Tianzhushania;(2) occurrence of abundant, 100-150μm,smooth-walled spherical microfossils;(3) occurrence of highly diverse acanthomorphic acritarchs including species extending from the second member and new forms in this member;(4) occurrence of unnamed new forms of protist;and(5) occurrence of the tubular microfossil Sinocyclocylicus guizhouensis.Since the Tianzhushania-dominated assemblage is not present in Australia,it seems that only the upper acanthomorph assemblage is present and thus the lower Doushantuo acanthomorph assemblage is missing in Australia. Large acanthomorphic acritarchs have been found in chert of the Ediacaran Doushantuo Formation at several localities in South China, including the East Yangtze Gorges of Hubei Province, Weng’an area of ​​Guizhou Province, and elsewhere. Host, their potential for biostratigraphic subdivision and correlation of Ediacaran successions is limited by facies control, taphonomic biases, and taxonomic problems. In the Yangtze Gorges, the Doushantuo Formation is generally subdivided into four lithologic members. However, in the Weng’an area, the Doushantuo Formation comprises just a lower and upper part separated by a mid-Doushantuo erosional surface.In the Yangtze Gorges at the Zhangcunping section, the Doushantuo succession is similar to that at Weng’an.So far, the correlation between the Yangtze Gorges and Weng’an area, and elsewhere has been an issue of debate. To resolve the debate, we selected eight sections in the Yangtze Gorges area and systemically sampled chert nodules of the Doushantuo Formation, fo cusing in particular on the upper Doushantuo Formation. Our data confirm two different assemblages appearing separately in the second and third members, which are separated by a negative δ ~ (13) C excursion (EN2). The lower assemblage is characterized by Tianzhushania and a diverse suite of large acanthomorphic acritarchs. The upper assemblage is distinguished from the lower assemblage, by (1) absence of Tianzhushania; (2) occurrence of abundant, 100-150μm, smooth- walled spherical microfossils; (3) occurrence of highly diverse acanthomorphic acritarchs including species extending from the second member and new forms in this member; (4) occurrence of unnamed new forms of protist; and (5) occurrence of the tubular microfossil Sinocyclocylicus guizhouensis.Since the Tianzhushania-dominated assemblage is not present in Australia, it seems that the upper the acanthomorph assemblage is present and thus the lower Doushantuo acanthomorph assemblage is missing in Australia.