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用反相高效液相色谱法测定中药复方制剂黄连上清片中有效成分盐酸小檗碱含量。采用Agilent 1100 ODS色谱柱(4.6mm×150mm,5μm),乙腈-0.05mol/L磷酸二氢钠溶液(用磷酸调pH值至3)(30∶70,V/V)为流动相,检测波长为345nm,流速1.0mL/min,柱温25℃。盐酸小檗碱在0.16—4.0μg/mL范围内与峰面积呈良好的线性,r=0.9999,平均加样回收率为98.96%(n=6),RSD=1.09%。该法快速简便,灵敏度和稳定性高,可有效控制黄连上清片的内在质量。
Determination of Berberine Hydrochloride in Chinese Herbal Prescriptions Coptis Chinensis by Reversed - Phase High Performance Liquid Chromatography. The mobile phase consisted of Agilent 1100 ODS column (4.6 mm × 150 mm, 5 μm), acetonitrile-0.05 mol / L sodium dihydrogen phosphate solution (adjusted to pH 3 with phosphoric acid) (30:70, V / V) 345nm, flow rate 1.0mL / min, column temperature 25 ℃. Berberine hydrochloride showed good linearity with the peak area in the range of 0.16-4.0 μg / mL, r = 0.9999. The average recovery was 98.96% (n = 6) and RSD was 1.09%. The method is fast and easy, high sensitivity and stability, which can effectively control the inner quality of Coptis on the clear film.