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目的 观察老龄小鼠异丙酚麻醉后对学习记忆及遗忘的影响。方法 选择正常昆明小鼠进行被动逃避避暗实验 ,分别于训练前 15min腹腔注射异丙酚 (5 0mg/kg和 10 0mg/kg)和训练后30min、3h腹腔注射异丙酚 (10 0mg/kg和 2 0 0mg/kg) ,记录训练后 2 4h的逃避电击进入暗室的潜伏期及 5min内的错误次数 ;处死动物 ,用放射免疫的方法检测小鼠脑中胆碱乙酰化酶的活性。结果 正常小鼠训练后 2 4h易于学习逃避电击进入暗室 ,潜伏期明显延长 ,给予小鼠 5 0mg/kg和 10 0mg/kg异丙酚潜伏期为 2 16± 0 2 7和 2 0 8± 0 36 ,与正常组比较明显缩短 (P <0 0 5 ,P <0 0 1) ;于训练后 30min即刻给予异丙酚 2 0 0mg/kg可出现逆行性遗忘 ,但于训练后 3h给药则未出现。异丙酚 (2 0 0mg/kg和10 0mg/kg)小鼠脑中胆碱乙酰化酶的活性于给药后 3h仍明显低于正常。结论 异丙酚可介导老年小鼠的顺行性和逆行性遗忘 ,可能与降低中枢胆碱能神经系统的活性有关
Objective To observe the effects of propofol anesthesia on learning, memory and forgetting in aged mice. Methods Normal Kunming mice were randomly assigned to escape from avoidance. Propofol (50 mg / kg and 10 mg / kg) and propofol (100 mg / kg) were injected intraperitoneally And 200 mg / kg). The latency of evading electric shock and the number of errors within 5 minutes after 24 h training were recorded. The animals were sacrificed and the activity of choline acetylase in brain was detected by radioimmunoassay. Results Normal mice were easy to learn to avoid electric shock and enter the darkroom 24 hours after training. The incubation period was significantly prolonged. The latency of 50 and 100 mg / kg of propofol given to mice was 216 ± 0 2 7 and 208 ± 0 36, Compared with the normal group, it was significantly shortened (P <0.05, P <0.01); retrograde amnesia was found at 200 mg / kg immediately after training 30 min, but not at 3 h after training . The activity of choline acetylase in the brain of propofol (200 mg / kg and 100 mg / kg) mice was still significantly lower than normal 3h after administration. Conclusion Propofol can mediate the anamnesis and retrograde amnesia in aged mice, which may be related to the decrease of central cholinergic nervous system activity