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通过继代草、日本看麦娘幼穗愈伤组织观察杂草对绿黄隆的诱导抗性特点。结果表明：继代过程中随着绿黄隆浓度逐渐增加，杂草愈伤组织内乙酰乳酸合酶（ＡＬＳ）对绿黄隆的敏感性（以ＩＣ５０值表示）明显下降，草愈伤组织经６～１２次继代，ＩＣ５０约从１μｇ／Ｌ增至３００μｇ／Ｌ；日本看麦娘愈伤组织经４～１０次继代，ＩＣ５０约从１μｇ／Ｌ增至１００μｇ／Ｌ以上。说明杂草愈伤组织对绿黄隆的诱导抗性明显增强，并且草的这种诱导抗性也在再生小植株上表达，表现为２００μｇ／Ｌ绿黄隆对抗性再生小植株的生长抑制减弱；１０００μｇ／Ｌ绿黄隆对抗性再生小植株的致死率明显变小。而在继代过程中绿黄隆并不显著改变这两种愈伤组织的生长状况，也不显著影响草愈伤组织的分化能力与再生小植株的发育
The seedlings of young spikelets were observed in Japan by observing the induced resistance of weeds to chlor-flavonoids. The results showed that with the increase of chlorothalonil concentration in the process of subculture, the sensitivity (by IC50 value) of acetolactate synthase (ALS) in weed callus to chlorflu49 was significantly decreased. The callus After 6 ~ 12 subcultures, the IC50 increased from 1μg / L to 300μg / L; in Japan, A. californica callus was subcultured 4 ~ 10 times with the IC50 increased from 1μg / L to above 100μg / L. The results showed that the induced resistance of weed callus to chloroplast was significantly enhanced, and this induced resistance of Humulus japonicus was also expressed on the regenerated plantlets, showing the growth inhibition of the chlorothalonine-resistant regenerated plantlets at 200μg / L Weakened. The lethal rate of 1000μg / L chlorflu49on resistant regenerative plantlets obviously decreased. In the process of subculture, Seculaculata did not significantly change the growth of the two callus, nor did it significantly affect the differentiation ability of the callus and the development of regenerated plantlets