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目的:探讨微小RNA-155(mi R-155)对急性脑梗死患者外周CD4+CD25+调节性T淋巴细胞(Treg)的调节作用,从而了解mi R-155在急性脑梗死发病中的作用机制。方法:选取南华大学附属南华医院神经内科和重症监护室急性脑梗死患者60例(轻度20例、中度20例、重度20例)及20例正常对照组。分别采集各组的外周静脉血,采用流式细胞术检测CD4+CD25+Treg细胞的表达;实时荧光定量聚合酶联反应(RT-PCR)检测mi R-155、Foxp3 m RNA的表达;酶联免疫吸附试验(ELISA)检测白细胞介素-10(IL-10)的水平。结果 :急性脑梗死组患者外周血Treg表达率和mi R-155、Foxp3 m RNA的表达以及IL-10水平均高于正常对照组(P<0.01);并且随着临床神经功能缺损程度评分增加而升高,重中轻度组之间两两比较差异均有统计学意义(均P<0.01);死亡组各项指标显著高于生存组(均P<0.01)。mi R-155的表达与Treg和Foxp3 m RNA的表达水平均呈正相关。结论:mi R-155参与对Treg细胞增殖的调节,在急性脑梗死免疫失衡中发挥一定的作用。
Objective: To investigate the regulatory effect of microRNA-155 (mi R-155) on peripheral CD4 + CD25 + regulatory T lymphocytes (Tregs) in patients with acute cerebral infarction so as to understand the mechanism of mi R-155 in the pathogenesis of acute cerebral infarction. Methods: Sixty patients (20 mild, 20 moderate, 20 severe) with acute cerebral infarction in Nanhua Hospital of Nanhua University and 20 normal controls were enrolled in this study. Peripheral venous blood was collected from each group, and the expression of CD4 + CD25 + Treg cells was detected by flow cytometry. The expression of mi R-155 and Foxp3 m RNA was detected by real-time fluorescence quantitative polymerase chain reaction (RT-PCR) The level of interleukin-10 (IL-10) was detected by ELISA. Results: The expression of Treg and the expression of mi R-155, Foxp3mRNA and IL-10 in peripheral blood of patients with acute cerebral infarction were higher than those of normal control group (P <0.01), and with the increase of clinical neurological deficit score (P <0.01). The indexes in death group were significantly higher than those in survival group (all P <0.01). The expression of mi R-155 was positively correlated with the expression of Treg and Foxp3 m RNA. Conclusion: mi R-155 is involved in the regulation of Treg cell proliferation and plays an important role in immune imbalance of acute cerebral infarction.