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利用荧光素及荧光素酶发光系统测定ATP的方法已广泛应用于各种生物化学研究以及工业、农业、医学等领域中。近几年来,国外已生产了多种利用荧光素酶系统测定ATP的仪器,并已商品化。如Lab-Line ATP光度计,Du Pont生物计、LKB生物发光计等。这些仪器操作简便、线性范围宽、灵敏度较高,可测10~(-14)克分子/毫升ATP变化。目前,测定荧光素酶系统发光技术可归为两类:一类是光子计数法,即在确定时间内计数(或积分)发光系统发射的光子数,这个方法比较方便,只要1台液体闪烁器就可进行测定。另一类是测定发光强度的方法,利用普通测定微电流的技术,仪器结构简单,容易制造,使用也方便。这个方法不仅可测定在确定时间内的发光强
The method of measuring ATP using fluorescein and luciferase luminescence system has been widely used in various biochemical research and industrial, agricultural, medical and other fields. In recent years, a variety of instruments have been produced abroad that measure ATP using the luciferase system and have been commercialized. Such as Lab-Line ATP photometer, Du Pont biometer, LKB bioluminescence meter and so on. These instruments are easy to operate, have a wide linear range and high sensitivity and can detect ATP changes of 10 to (-14) mol / ml. At present, the determination of luciferase system luminescence technology can be grouped into two categories: one is the photon counting method, that is counted within a certain time (or integral) the number of photons emitted by the light-emitting system, this method is more convenient, as long as a liquid scintillator Can be measured. The other is to measure the luminous intensity of the method, the use of ordinary micro-current measurement technology, the instrument structure is simple, easy to manufacture, easy to use. This method can not only determine the luminous intensity in a certain time