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木薯是一种重要的能源和淀粉作物,具有较强的抗逆性。为研究木薯中决定细胞分裂素合成的IPT基因如何被逆境信号调控,从木薯基因组数据库中获得了两个Me IPT基因,序列分析显示两者启动子区同时具有茉莉酸和乙烯响应元件。以木薯品种“华南八号”悬浮培养细胞为材料,利用q RT-PCR检测木薯Me IPT1和Me IPT2基因在乙烯利和茉莉酸甲酯处理后的表达模式。结果显示茉莉酸甲酯处理后两个基因显著上调,但乙烯信号对其的影响却不尽相同。这些数据说明这两个Me IPT基因可被乙烯和茉莉酸信号调控,并推测其在进一步影响细胞分裂素的生物合成中可能受整合后的不同激素信号调控。
Cassava is an important source of energy and starch crops, with strong resistance. To investigate how IPT genes that determine cytokinin synthesis in cassava are regulated by stress signals, two Me IPT genes were obtained from the cassava genome database. Sequence analysis revealed that both IPT genes have jasmonic acid and ethylene response elements in their promoter regions. The camptothecin expression patterns of Me IPT1 and Me IPT2 in cauliflower and “Hua Nan No.8” suspension culture cells were detected by q RT-PCR after treatment with ethephon and methyl jasmonate. The results showed that the two genes were significantly up-regulated after methyl jasmonate treatment, but the effect of ethylene signal on them was different. These data indicate that these two Me IPT genes are regulated by ethylene and jasmonic acid signals and speculated that they may be regulated by different hormonal signals after integration in the biosynthesis that further affects cytokinins.