转双价基因(Cry1 Ac+CpTI)抗虫棉(ZGK 9 71 2)对小地老虎抗虫性研究

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室内测定了转 Cry1 Ac基因抗虫棉和转双价基因 (Cry1 Ac+Cp TI)抗虫棉对小地老虎的存活、生长发育、繁殖及营养效应的影响。结果表明 ,两种转基因抗虫棉对小地老虎幼虫均有一定的抗性 ,转 Cry1 Ac基因抗虫棉对低龄幼虫的抗性好于转双价基因抗虫棉 ,而转双价基因抗虫棉对高龄幼虫的抗性好于转 Cry1 Ac基因抗虫棉 ,但两种转基因抗虫棉对小地老虎幼虫的控制作用均不理想。两种转基因抗虫棉对小地老虎幼虫的生长发育、繁殖和营养效应均有较大的影响。表现为发育历期延长 ,虫重和蛹重减轻 ,羽化率、化蛹率、产卵量和卵孵化率降低 ;相对生长率、相对取食量、近似消化率、食物转化率和利用率明显降低 ,而相对代谢速率明显增加。转双价基因抗虫棉的影响更为明显 The effects of Bt transgenic Cry1 Ac + CpTI insect-resistant cotton on the survival, growth, reproductive and nutritional effects of A. tabaci were determined indoors. The results showed that both of the transgenic Bt cotton had some resistance to the Tigers larvae. Cry1Ac transgenic cotton had lower resistance to young larvae than Bt transgenic cotton, Cotton bollworm resistance to aging larvae was better than that of Cry1Ac transgenic cotton, but the control effects of the two transgenic cottons on small-larvae larvae were not satisfactory. Both transgenic Bt cotton had a greater impact on the growth, reproduction and nutrition effects of Larvae Larvae. The results showed that the developmental duration was prolonged, the insect weight and pupal weight were reduced, the emergence rate, pupation rate, egg yield and egg hatching rate were decreased. The relative growth rate, relative food intake, approximate digestibility, food conversion rate and utilization rate were significantly Decreased, while the relative metabolic rate increased significantly. The effect of transgenic cotton with Bt gene is more obvious
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