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目的 研究热盐水灌胃导致的大鼠萎缩性胃炎胃粘膜组织中的凋亡细胞及增殖细胞表达状态 ,以探讨长期热咸饮食与慢性萎缩性胃炎发生的关系。方法 采用脱氧核糖核酸转移酶介导的缺口末端标记 (TUNEL)技术及免疫组织化学染色技术。细胞凋亡时DNA含量分析采用流式细胞检测技术。结果 ①TUNEL检测细胞凋亡显示 :在热盐水所致的大鼠萎缩性胃炎中凋亡细胞数明显增多 ,在粘膜全层均可见到 ,呈弥漫性分布。萎缩性胃炎中凋亡细胞指数显著高于正常大鼠胃粘膜 (P <0 .0 5) ;②免疫组化法检测显示 :增殖细胞核抗原 (PCNA)在热盐水灌胃所致的萎缩性胃炎中的表达率显著高于正常胃粘膜 (P <0 .0 5) ;③流式细胞检测显示 :萎缩性胃炎时 ,在G0 /G1峰前可见一个亚G0 /G1峰 ,也即凋亡细胞峰出现 ,而在正常胃粘膜时未显示亚G0 /G1峰。结论 热盐水灌胃可导致大鼠胃粘膜组织细胞增殖和细胞凋亡异常 ,其在热盐水所致的大鼠萎缩性胃炎发生中起重要作用。
Objective To study the expression of apoptotic cells and proliferating cells in gastric mucosa of rats with atrophic gastritis caused by intragastric administration of hot saline in order to investigate the relationship between chronic hot and salty diets and the occurrence of chronic atrophic gastritis. Methods TdT - mediated nick end labeling (TUNEL) and immunohistochemical staining were used. DNA content analysis of apoptotic cells using flow cytometry. Results ① The apoptosis of TUNEL assay showed that the number of apoptotic cells in atrophic gastritis induced by hot saline was significantly increased, which was found in all layers of mucosa and showed diffuse distribution. The index of apoptotic cells in atrophic gastritis was significantly higher than that in normal rat gastric mucosa (P <0.05). ② Immunohistochemical staining showed that proliferative cell nuclear antigen (PCNA) was atrophic gastritis (P <0.05). ③Flow cytometry showed a sub-G0 / G1 peak before G0 / G1 peak in atrophic gastritis, that is, apoptotic cells Peaks appeared, while in normal gastric mucosa, no sub-G0 / G1 peaks were shown. Conclusion Hot salt water can induce gastric mucosal cell proliferation and apoptosis in rats, which play an important role in the pathogenesis of atrophic gastritis induced by hot salt water.