背景:脂褐素的沉积、超氧化物歧化酶下降和丙二醛升高是衰老的重要表现,一些研究提示。中草药在延缓衰老方面具有明显的优势。目的:观察茯苓生脉饮药物血清对离体大鼠脑海马神经元超氧化物歧化酶、丙二醛及脂褐素水平的影响,并与维生素E血清及空白血清作对照。设计:随机对照实验。单位:华中科技大学同济医学院实验动物学部,华中科技大学同济医学院解剖教研室细胞培养中心,武汉市中医医院医科所。材料:实验于2000-10/2001-2在华中科技大学同济医学院实验动物学部及华中科技大学同济医学院解剖教研室细胞培养中心完成。选用健康日本大耳白兔6只,兔龄不限,雄性,常规饲养,体质量(2.5±0.1)kg,随机分成3组,每组2只;清洁级SD大鼠30只,新生1～2d,雌雄不拘。方法:①将大鼠脑海马神经悬液,按2.5×108/L密度接种至24孔培养板各孔中培养。将每板各孔培养细胞随机分成3组,即茯苓生脉饮组(茯苓生脉饮由茯苓、人参、麦冬、五味子等中药制成,每毫升含生药1.5g)、药物对照组(维生素E)和空白对照组,每组8孔。24h后,茯苓生脉饮组、药物对照组、空白对照组所在培养板每孔分别加入茯苓生脉饮含药血清、维生素E含药血清和空白血清1mL。②在细胞培养的第6天加入黄嘌呤,一次黄嘌呤反应体系致细胞损伤,产生超氧阴离子自由基,成为衰老模型。③在培养过程中,每次更换培养液时,各组培养孔中均需重新分别加入上述相应的药液。④培养至第15天时,检测各组脑海马神经细胞超氧化物歧化酶、丙二醛、脂褐素水平。主要观察指标:各组大鼠脑海马神经元超氧化物歧化酶、丙二醛及脂褐素水平。结果:茯苓生脉饮组脑海马神经细胞超氧化物歧化酶水平显著高于空白对造组,丙二醛水平低于药物对照组,脂褐素水平低于空白对照组(P<0.05)。结论:茯苓生脉饮中主要成分茯苓、人参、麦冬、五味子各单味药均有抗氧化清除自由基的作用,茯苓生脉饮能提高超氧化物歧化酶的活性,降低丙二醛的含量,抑制脂褐素的形成,从而延缓衰老的进程。 Background: The deposition of lipofuscin, the decrease of superoxide dismutase and the elevation of malondialdehyde are important manifestations of aging. Some studies suggest that. Chinese herbal medicine has obvious advantages in delaying aging. OBJECTIVE: To observe the effect of Qishengmaiyin serum on the levels of superoxide dismutase, malondialdehyde and lipofuscin in isolated hippocampal neurons of rats, and compare it with vitamin E serum and blank serum. Design: Randomized controlled trials. Unit: Department of Laboratory Zoology, Tongji Medical College, Huazhong University of Science and Technology, Department of Anatomy, Cell Culture Center, Tongji Medical College, Huazhong University of Science and Technology, Institute of Medicine, Wuhan Traditional Chinese Medicine Hospital. MATERIALS: The experiment was performed at the Cell Culture Center of Department of Laboratory Zoology, Tongji Medical College, Huazhong University of Science and Technology, and Department of Anatomy, Tongji Medical College, Huazhong University of Science and Technology from October 2000 to October 2001. Six healthy Japanese white rabbits were selected. The rabbits were male, and the males were fed regularly. The body weight was (2.5±0.1) kg. They were randomly divided into 3 groups with 2 rats in each group. 2d, either male or female. METHODS: 1 Rat brain hippocampal nerve suspension was inoculated into each well of a 24-well culture plate at a density of 2.5×108/L. The cultured cells in each well of each plate were randomly divided into 3 groups, ie, Dishengmai Yin group (Zhengshengyin made from Chinese medicine, Ginseng, Ophiopogon japonicus, Schisandra, etc., each containing 1.5g of crude drug), and the drug control group (vitamin E) and blank control groups, 8 wells per group. Twenty-four hours later, the Shengshengmai Yin-containing serum, vitamin E-containing serum and blank serum were added to each well of the Shengshengmai group, drug control group, and blank control group. 2 Xanthine was added on the 6th day of cell culture, and the xanthine reaction system caused cell damage, resulting in superoxide anion free radicals and becoming an aging model. 3 During the culture process, each time the culture fluid is changed, each group of culture wells must be re-incorporated with the above-mentioned corresponding chemical solutions. 4 On the 15th day of culture, the levels of superoxide dismutase, malondialdehyde, and lipofuscin were measured in brain cells of each group. MAIN OUTCOME MEASURES: Superoxide dismutase, malondialdehyde and lipofuscin levels in brain hippocampal neurons of rats in each group. RESULTS: The SOD level in hippocampal neurons of Gengmaiyin group was significantly higher than that of blank control group. The level of malondialdehyde was lower than that of drug control group, and lipofuscin level was lower than that of blank control group (P<0.05). Conclusion: The main components of Qishengmaiyin, including wolfberry, ginseng, Ophiopogon japonicus and Schisandra chinensis, all have single anti-oxidation and anti-scavenging effects, and Shengmaiyin can increase the activity of superoxide dismutase and decrease the content of malondialdehyde. Content, inhibit the formation of lipofuscin, thereby delaying the aging process.