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旨在研究碱性成纤维细胞生长因子(bFGF)对兔骨髓间充质干细胞(BMSCs)体外生长及增殖的影响。体外培养并鉴定兔BMSCs,用不同浓度的bFGF(5、10、20、40、80和100μg.L-1)作用于兔BMSCs,用四甲基偶氮唑盐比色法(MTT)、流式细胞仪观察细胞的生长及增殖情况。结果,经免疫细胞化学法和分化反推法鉴定所分离培养的细胞为兔骨髓间充质干细胞;MTT法结果显示,bFGF浓度为80μg.L-1时细胞的增殖能力最强(P<0.01),在培养第3天即出现显著的促增殖作用(P<0.01);流式细胞仪结果显示,bFGF组BMSCs的增殖指数明显高于对照组(P<0.01)。说明bFGF有促进体外培养兔BMSCs增殖的作用,浓度为80μg.L-1时促增殖能力最强,可以作为体外扩增培养兔骨髓间充质干细胞的最佳培养条件。
To investigate the effects of basic fibroblast growth factor (bFGF) on the growth and proliferation of rabbit bone marrow mesenchymal stem cells (BMSCs) in vitro. Rabbit BMSCs were cultured and identified in vitro. Rabbit BMSCs were treated with different concentrations of bFGF (5, 10, 20, 40, 80 and 100μg.L-1), MTT, Cytometry observed cell growth and proliferation. The results showed that BMSCs were identified by immunocytochemistry and differentiation-back analysis. MTT assay showed that the proliferation of cells was the strongest when the concentration of bFGF was 80μg.L-1 (P <0.01) ). The proliferation of BMSCs in bFGF group was significantly higher than that in control group (P <0.01) by flow cytometry. The results showed that bFGF could promote the proliferation of rabbit BMSCs in vitro. When the concentration of bFGF was 80μg.L-1, the strongest proliferative ability could be used as the best culture condition for expanding rabbit BMSCs.